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IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit TLR5 Polyclonal Antibody | anti-TLR5 antibody
Phospho-TLR5 (Tyr798) Antibody
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human, MousePredicted Reactivity: Pig (88%), Rabbit (88%), Chicken (100%), Xenopus (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
TLR5, Antibody; Phospho-TLR5 (Tyr798) Antibody; FLJ10052; MGC126430; MGC126431; SLEB1; TIL 3; TIL3; TLR 5; Tlr5; TLR5_HUMAN; Toll like receptor 5; Toll like receptor 5 precursor; Toll-like receptor 5; Toll/interleukin 1 receptor like protein 3; Toll/interleukin-1 receptor-like protein 3; anti-TLR5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (88%), Rabbit (88%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (88%), Rabbit (88%), Chicken (100%), Xenopus (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-TLR5 (Tyr798) Antibody detects endogenous levels of TLR5 only when phosphorylated at Tyr798.
Tissue Specificity: Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.
Tissue Specificity: Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TLR5 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TLR5 around the phosphorylation site of Tyr798.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
Subunit Structure
Homodimer. Interacts with MYD88 (via TIR domain). Interacts with TICAM1 (via TIR domain). Interacts with UNC93B1; this interaction is essential for proper TLR5 localization to the plasma membrane.
Similarity
Belongs to the Toll-like receptor family.
Subcellular Location
Cell membrane>Single-pass type I membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of TLR5 (Phospho-Tyr798) using PMA treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of TLR5 (Phospho-Tyr798) using PMA treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from 3T3-L1P6(heat shock treatment), using Phospho-TLR5 (Tyr798) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 3T3-L1P6(heat shock treatment), using Phospho-TLR5 (Tyr798) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TLR5 antibody
Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
858
NCBI Official Full Name
Toll-like receptor 5
NCBI Official Synonym Full Names
toll-like receptor 5
NCBI Official Symbol
TLR5
NCBI Official Synonym Symbols
SLE1; TIL3; SLEB1; MELIOS
NCBI Protein Information
toll-like receptor 5; toll/interleukin-1 receptor-like protein 3; systemic lupus erythematosus susceptibility 1
UniProt Protein Name
Toll-like receptor 5
UniProt Gene Name
TLR5
UniProt Synonym Gene Names
TIL3
UniProt Entry Name
TLR5_HUMAN
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Product Notes
The TLR5 tlr5 (Catalog #AAA329844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TLR5 (Tyr798) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (88%), Rabbit (88%), Chicken (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's TLR5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TLR5 tlr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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