Rabbit TMEM106B Polyclonal Antibody | anti-TMEM106B antibody

TMEM106B Antibody (C-Term)

Cat. Num. AAA290848

• Reactivity: Human, Mouse; Predicted: Bovine, Rat
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Synonyms: TMEM106B, Antibody; TMEM106B Antibody (C-Term); Transmembrane protein 106B; TMEM106B; anti-TMEM106B antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This TMEM106B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 218-252 amino acids from human TMEM106B.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 274

• Applicable Applications for anti-TMEM106B antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: In neurons, involved in the transport of late endosomes/lysosomes (PubMed:25066864). May be involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking (PubMed:25066864). May act as a molecular brake for retrograde transport of late endosomes/lysosomes, possibly via its interaction with MAP6 (By similarity). In motoneurons, may mediate the axonal transport of lysosomes and axonal sorting at the initial segment (By similarity). It remains unclear whether TMEM106B affects the transport of moving lysosomes in the anterograde or retrograde direction in neurites and whether it is important in the sorting of lysosomes in axons or in dendrites (By similarity). In neurons, may also play a role in the regulation of lysosomal size and responsiveness to stress (PubMed:25066864). Required for proper lysosomal acidification (By similarity).
• Cellular Location: Late endosome membrane; Single-pass type II membrane protein. Lysosome membrane; Single-pass type II membrane protein. Membrane; Lipid-anchor
• Tissue Location: Expressed in frontal cortex.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Overlay histogram showing Hela cells stained with AAA290848(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290848, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

WB (Western Blot)

(Anti-TMEM106B Antibody (C-Term) at 1:2000 dilution + Mouse brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-TMEM106B antibody

Primary Antibodies; Neuroscience

References

Ota T.,et al.Nat. Genet. 36:40-45 (2004). Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Scherer S.W.,et al.Science 300:767-772 (2003). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Hillier L.W.,et al.Nature 424:157-164 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 197116389
• NCBI GeneID: 54664
• NCBI Accession #: NP_001127704.1
• NCBI GenBank Nucleotide #: NM_001134232.1
• UniProt Accession #: Q9NUM4; Q53FL9; Q8N4L0; A4D108
• Molecular Weight: 31127
• NCBI Official Full Name: transmembrane protein 106B
• NCBI Official Synonym Full Names: transmembrane protein 106B
• NCBI Official Symbol: TMEM106B
• NCBI Protein Information: transmembrane protein 106B
• UniProt Protein Name: Transmembrane protein 106B
• UniProt Gene Name: TMEM106B