<
IF (Immunofluorescence)
(Immunofluorescence staining of TMEM38A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TMEM38A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit TMEM38A Polyclonal Antibody | anti-TMEM38A antibody
Anti-TMEM38A Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TMEM38A, Antibody; Anti-TMEM38A Antibody, Rabbit Polyclonal; TMEM38A Antibody, Rabbit PAb, Antigen Affinity Purified; transmembrane protein 38A; Anti-TRIC-A Antibody; Anti-TRICA Antibody; anti-TMEM38A antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMEM38A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TMEM38A antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human TMEM38A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TMEM38A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of TMEM38A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TMEM38A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of TMEM38A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TMEM38A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining of human TMEM38A in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human TMEM38A in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMEM38A in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TMEM38A in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-TMEM38A rabbit polyclonal antibody at 1:5000 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa)
WB (Western Blot)
(Anti-TMEM38A rabbit polyclonal antibody at 1:5000 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa)
Related Product Information for anti-TMEM38A antibody
TMEM38A (Transmembrane Protein 38A) is a Protein Coding gene. The TMEM38A gene, located on 19p13.11, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. An important paralog of this gene is TMEM38B. TMEM38A is a monovalent cation channel required for the maintenance of rapid intracellular calcium release and may act as a potassium counter-ion channel that functions in synchronization with calcium release from intracellular stores. TMEM38A is widely expressed in the brain, stomach, and other tissues. Diseases associated with TMEM38A include Hyperalphalipoproteinemia 1 and Myopathy, X-Linked, With Postural Muscle Atrophy. 236 organisms have orthologs with the human gene TMEM38A.
References
Yamazaki D, et al. (2009) New molecular components supporting ryanodine receptor-mediated ca(2+) release: Roles of junctophilin and tric channel in embryonic cardiomyocytes. Pharmacol Ther 121 (3): 265-272.Shrestha N, et al. (2020) Tric-a shapes oscillatory ca2+ signals by interaction with stim1/orai1 complexes. PLoS Biol 18 (4): e3000700.Bailey SD, et al. (2010) Variation at the nfatc2 locus increases the risk of thiazolidinedione-induced edema in the diabetes reduction assessment with ramipril and rosiglitazone medication (dream) study. Diabetes Care 33 (10): 2250-2253.
Similar Products
Product Notes
The TMEM38A (Catalog #AAA256301) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMEM38A Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM38A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TMEM38A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM38A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.