FCM/FACS (Flow Cytometry)
(Applications: Flow CytometrySample: THP-1 CellsDetection: BD FACSCalibur"Under these experimental conditions...[TNF-alpha P28 anti-CD14] demonstrated positive staining of the THP-1 cells.")
anti-Mouse TNF alpha Polyclonal Antibody | anti-TNF alpha antibody
Anti-TNF alpha Antibody
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Reactivity
Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
TNF alpha, Antibody; Anti-TNF alpha Antibody; Tumor necrosis factor; APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form; Tumour Necrosis Factor Alpha; tumor necrosis factor; anti-TNF alpha antibody
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
219
Applicable Applications for anti-TNF alpha antibody
WB (Western Blot)
Immunogen
E Coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Applications: Flow CytometrySample: THP-1 CellsDetection: BD FACSCalibur"Under these experimental conditions...[TNF-alpha P28 anti-CD14] demonstrated positive staining of the THP-1 cells.")
FCM/FACS (Flow Cytometry)
(Applications: Flow CytometrySample: THP-1 CellsDetection: BD FACSCalibur"Under these experimental conditions...[TNF-alpha P28 anti-CD14] demonstrated positive staining of the THP-1 cells.")
WB (Western Blot)
(Anti- TNF alpha Picoband antibody, AAA46300, Western blottingAll lanes: Anti TNF alpha (AAA46300) at 0.5ug/mlLane 1: Mouse Kidney Tissue Lysate at 50ugLane 2: Mouse Intestine Tissue Lysate at 50ugPredicted bind size: 25KDObserved bind size: 25KD)
WB (Western Blot)
(Anti- TNF alpha Picoband antibody, AAA46300, Western blottingAll lanes: Anti TNF alpha (AAA46300) at 0.5ug/mlLane 1: Mouse Kidney Tissue Lysate at 50ugLane 2: Mouse Intestine Tissue Lysate at 50ugPredicted bind size: 25KDObserved bind size: 25KD)
WB (Western Blot)
(Anti- TNF alpha Picoband antibody, AAA46300, Western blottingAll lanes: Anti TNF alpha (AAA46300) at 0.5ug/mlWB: Recombinant Mouse TNF alpha Protein 0.5ngPredicted bind size: 15KDObserved bind size: 15KD)
WB (Western Blot)
(Anti- TNF alpha Picoband antibody, AAA46300, Western blottingAll lanes: Anti TNF alpha (AAA46300) at 0.5ug/mlWB: Recombinant Mouse TNF alpha Protein 0.5ngPredicted bind size: 15KDObserved bind size: 15KD)
Related Product Information for anti-TNF alpha antibody
Description: Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB in Mouse.
Background: TNFalpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Background: TNFalpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
References
1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985. 2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,896 Da
NCBI Official Full Name
tumor necrosis factor isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
Tnf
NCBI Official Synonym Symbols
DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
Tnf
UniProt Synonym Gene Names
Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_MOUSE
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Product Notes
The TNF alpha tnf (Catalog #AAA46300) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNF alpha Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNF alpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TNF alpha tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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