Rabbit anti-Human TP53 Polyclonal Antibody | anti-TP53 antibody

TP53 Antibody

Cat. Num. AAA301607

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TP53, Antibody; TP53 Antibody; TP53; FLJ92943; LFS1; TRP53; p53; anti-TP53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TP53 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-TP53 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TP53.
• Target Name: TP53
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cell using TP53 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using TP53 antibody.)

Related Product Information for anti-TP53 antibody

The TP53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of TP53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). TP53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of TP53 at Ser15 and Ser20 and leads to a reduced interaction between TP53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits TP53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). TP53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind TP53, promoting both the accumulation and activation of TP53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate TP53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). TP53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of TP53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of TP53 (10,13,14). TP53 is phosphorylated at Ser6 and Ser9 by CK1d and CK1eta both in vitro and in vivo (13,15). Phosphorylation of TP53 at Ser46 regulates the ability of TP53 to induce apoptosis (16). Acetylation of TP53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes TP53. Acetylation appears to play a positive role in the accumulation of TP53 protein in stress response (17).

Product Categories/Family for anti-TP53 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 120407068
• NCBI GeneID: 7157
• NCBI Accession #: NP_000537.3
• NCBI GenBank Nucleotide #: NM_000546.5
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98
• Molecular Weight: 24,401 Da
• NCBI Official Full Name: cellular tumor antigen p53 isoform a
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN