IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HT-29 cells treated by nocodazole using 2.5ug Phospho-TP53-S33 antibody. Western blot was performed from the immunoprecipitate using Phospho-TP53-S33 antibody at a dilition of 1:1000.)
Rabbit anti-Human TP53-S33 Polyclonal Antibody | anti-TP53-S33 antibody
Phospho-TP53-S33 pAb
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purification
Synonyms
TP53-S33, Antibody; Phospho-TP53-S33 pAb; BCC7; LFS1; P53; TRP53; anti-TP53-S33 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
VLSPL
Sequence Length
393
Applicable Applications for anti-TP53-S33 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding S33 of human TP53
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HT-29 cells treated by nocodazole using 2.5ug Phospho-TP53-S33 antibody. Western blot was performed from the immunoprecipitate using Phospho-TP53-S33 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HT-29 cells treated by nocodazole using 2.5ug Phospho-TP53-S33 antibody. Western blot was performed from the immunoprecipitate using Phospho-TP53-S33 antibody at a dilition of 1:1000.)
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-TP53-S33 antibody
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Product Categories/Family for anti-TP53-S33 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 23-43kDa
Observed: 53kDa
Observed: 53kDa
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
Similar Products
Product Notes
The TP53-S33 tp53 (Catalog #AAA281281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TP53-S33 pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TP53-S33 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TP53-S33 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VLSPL. It is sometimes possible for the material contained within the vial of "TP53-S33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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