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WB (Western Blot)
(All lanes : Anti-TPPP3 Antibody (C-term) at 1:2000 dilutionLane 1: WiDr whole cell lysateLane 2: Mouse cerebellum lysateLane 3: Mouse skeletal muscle lysateLane 4: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit TPPP3 Polyclonal Antibody | anti-TPPP3 antibody
TPPP3 Antibody (C-term)
Gene Names
TPPP3; p20; CGI-38; TPPP/p20; p25gamma
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
TPPP3, Antibody; TPPP3 Antibody (C-term); Tubulin polymerization-promoting protein family member 3; TPPP/p20; TPPP3; anti-TPPP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This TPPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human TPPP3.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
117-146
Sequence Length
176
Applicable Applications for anti-TPPP3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Regulator of microtubule dynamic that has microtubule bundling activity (PubMed:17105200, PubMed:19633818). Required for embryo implantation; possibly by regulating beta-catenin (By similarity). Also required for decidualization via regulation of beta- catenin (PubMed:30667362).
Cellular Location
Cytoplasm. Cytoplasm, cytoskeleton
Tissue Location
Expressed in endometrium during the mid-secretory phase (LH + 7) (at protein level).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-TPPP3 Antibody (C-term) at 1:2000 dilutionLane 1: WiDr whole cell lysateLane 2: Mouse cerebellum lysateLane 3: Mouse skeletal muscle lysateLane 4: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-TPPP3 Antibody (C-term) at 1:2000 dilutionLane 1: WiDr whole cell lysateLane 2: Mouse cerebellum lysateLane 3: Mouse skeletal muscle lysateLane 4: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(TPPP3 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TPPP3 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(TPPP3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(TPPP3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
((LEFT)Western blot analysis of TPPP3 Antibody (C-term) in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
((LEFT)Western blot analysis of TPPP3 Antibody (C-term) in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-TPPP3 antibody
TPPP3 binds tubulin and has microtubule bundling activity. TPPP3 may play a role in cell proliferation and mitosis.
Product Categories/Family for anti-TPPP3 antibody
References
Zhou, W., et al. Mol. Cell. Biochem. 333 (1-2), 91-98 (2010) Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009) Vincze, O., et al. Biochemistry 45(46):13818-13826(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18985
NCBI Official Full Name
tubulin polymerization-promoting protein family member 3
NCBI Official Synonym Full Names
tubulin polymerization-promoting protein family member 3
NCBI Official Symbol
TPPP3
NCBI Official Synonym Symbols
p20; CGI-38; TPPP/p20; p25gamma
NCBI Protein Information
tubulin polymerization-promoting protein family member 3
UniProt Protein Name
Tubulin polymerization-promoting protein family member 3
UniProt Gene Name
TPPP3
UniProt Entry Name
TPPP3_HUMAN
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Product Notes
The TPPP3 tppp3 (Catalog #AAA287824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 117-146. The TPPP3 Antibody (C-term) reacts with Human, Mouse, Rat (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's TPPP3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TPPP3 tppp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPPP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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