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product-image-AAA212040_IP13.jpg IP (Immunoprecipitation) (Detection of human TRAF4 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAF4 antibody AAA212040 (lot AAA212040-2) used for IP at 3 ug per reaction. TRAF4 was also immunoprecipitated by rabbit anti-TRAF4 antibody For blotting immunoprecipitated TRAF4, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human TRAF4 Polyclonal Antibody | anti-TRAF4 antibody

Rabbit anti-TRAF4 Antibody, Affinity Purified

Gene Names
TRAF4; CART1; MLN62; RNF83
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
TRAF4, Antibody; Rabbit anti-TRAF4 Antibody, Affinity Purified; cysteine-rich domain associated with ring and TRAF domain; RING finger protein 83; TRAF4 variant 6; TNF receptor-associated factor 4; tumor necrosis receptor-associated factor 4A; RNF83; MLN62; MLN 62; metastatic lymph node gene 62 protein; cysteine-rich domain associated with RING and Traf domains protein 1; CART1; malignant 62; TNF receptor associated factor 4; anti-TRAF4 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TRAF4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 420 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

IP (Immunoprecipitation)

(Detection of human TRAF4 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAF4 antibody AAA212040 (lot AAA212040-2) used for IP at 3 ug per reaction. TRAF4 was also immunoprecipitated by rabbit anti-TRAF4 antibody For blotting immunoprecipitated TRAF4, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212040_IP13.jpg IP (Immunoprecipitation) (Detection of human TRAF4 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAF4 antibody AAA212040 (lot AAA212040-2) used for IP at 3 ug per reaction. TRAF4 was also immunoprecipitated by rabbit anti-TRAF4 antibody For blotting immunoprecipitated TRAF4, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry)

(Detection of human TRAF4 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-TRAF4 (Cat. No. AAA212040 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212040_IHC15.jpg IHC (Immunohistochemistry) (Detection of human TRAF4 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-TRAF4 (Cat. No. AAA212040 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-TRAF4 antibody
TNF receptor-associated factor 4 (TRAF4) is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. TRAF4 seems to mediate activation of NF-kappa-B and JNK and to be involved in apoptosis. TRAF4 may play a role in the development of respiratory tract. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BUZ4].
Product Categories/Family for anti-TRAF4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,543 Da
NCBI Official Full Name
TNF receptor-associated factor 4
NCBI Official Synonym Full Names
TNF receptor-associated factor 4
NCBI Official Symbol
TRAF4
NCBI Official Synonym Symbols
CART1; MLN62; RNF83
NCBI Protein Information
TNF receptor-associated factor 4; MLN 62; malignant 62; RING finger protein 83; metastatic lymph node gene 62 protein; tumor necrosis receptor-associated factor 4A; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated
UniProt Protein Name
TNF receptor-associated factor 4
UniProt Gene Name
TRAF4
UniProt Synonym Gene Names
CART1; MLN62; RNF83; MLN 62
UniProt Entry Name
TRAF4_HUMAN

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Product Notes

The TRAF4 traf4 (Catalog #AAA212040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TRAF4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the TRAF4 traf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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