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IP (Immunoprecipitation)
(TRAF4 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TRAF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TRAF4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
Rabbit anti-Human, Mouse TRAF4 Polyclonal Antibody | anti-Traf4 antibody
Anti-TRAF4 Antibody, Rabbit Polyclonal
Gene Names
TRAF4; CART1; MLN62; RNF83
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
TRAF4, Antibody; Anti-TRAF4 Antibody, Rabbit Polyclonal; Traf4 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-CART1 Antibody; Anti-MLN62 Antibody; Anti-RNF83 Antibody; anti-Traf4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TRAF4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Traf4 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Traf4
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Traf4, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(TRAF4 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TRAF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TRAF4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
IP (Immunoprecipitation)
(TRAF4 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TRAF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TRAF4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of TRAF4 in U2OS and HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TRAF4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of TRAF4 in U2OS and HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TRAF4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining TRAF4 in mouse kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemisry)
(Immunochemical staining TRAF4 in mouse kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining TRAF4 in human lung cancer with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining TRAF4 in human lung cancer with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining TRAF4 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining TRAF4 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-Traf4 antibody
traf4a mRNA is expressed in the developing eye vesicle over the period of optic cup morphogenesis, and Traf4a loss leads to disrupted evagination and elongation of the eye vesicles, and aberrant organization and apico-basal polarity of the eye epithelium. Traf4 acts in a TNF receptor-and Jun N-terminal protein kinase (JNK)-independent manner to fine-tune the assembly of adherens junctions in the invaginating mesodermal cells. Tumor necrosis factor receptor associated factor 4 (Traf4) mRNA expression is upregulated in various breast tumors and tumor cell lines.
Product Categories/Family for anti-Traf4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,543 Da
NCBI Official Full Name
TNF receptor-associated factor 4
NCBI Official Synonym Full Names
TNF receptor-associated factor 4
NCBI Official Symbol
TRAF4
NCBI Official Synonym Symbols
CART1; MLN62; RNF83
NCBI Protein Information
TNF receptor-associated factor 4; MLN 62; malignant 62; RING finger protein 83; metastatic lymph node gene 62 protein; tumor necrosis receptor-associated factor 4A; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated
UniProt Protein Name
TNF receptor-associated factor 4
UniProt Gene Name
TRAF4
UniProt Synonym Gene Names
CART1; MLN62; RNF83; MLN 62
UniProt Entry Name
TRAF4_HUMAN
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Product Notes
The Traf4 traf4 (Catalog #AAA254373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRAF4 Antibody, Rabbit Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Traf4 traf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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