WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with tri-methyl-histone H3 (Lys9) antibody (AAA50474) at a 1 ug/ml dilution.)
Rabbit anti-Human Tri-Methyl-Histone H3 Polyclonal Antibody | anti-HIST1H3F antibody
Tri-Methyl-Histone H3 (Lys9) antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Antiserum to tri-methyl-histone H3 (Lys9) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Synonyms
Tri-Methyl-Histone H3, Antibody; Tri-Methyl-Histone H3 (Lys9) antibody; RABBIT ANTI TRI-METHYL-HISTONE H3 (Lys9); anti-HIST1H3F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to tri-methyl-histone H3 (Lys9) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-HIST1H3F antibody
WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation)
Immunogen
A peptide including tri-methylated lysine 9 of histone H3
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with tri-methyl-histone H3 (Lys9) antibody (AAA50474) at a 1 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with tri-methyl-histone H3 (Lys9) antibody (AAA50474) at a 1 ug/ml dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with tri-methyl-histone H3 (Lys9) antibody (AAA50474) at a 1 ug/ml dilution (top) and DAPI (middle). Bottom panel shows merged images.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with tri-methyl-histone H3 (Lys9) antibody (AAA50474) at a 1 ug/ml dilution (top) and DAPI (middle). Bottom panel shows merged images.)
Related Product Information for anti-HIST1H3F antibody
Rabbit anti Human tri-methyl-histone H3 (Lys9) antibody recognizes histone H3 when tri-methylated at lysine 9.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression (Gupta et al. 2010). Lysine 9 of histone H3 may be mono-, di- or tri-methylated by SuvH39H1 or G9a, usually leading to transcriptional repression (Lachner et al. 2001).
Wide species cross-reactivity is expected from Rabbit anti Human tri-methyl-histone H3 (Lys9) antibody based on sequence.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression (Gupta et al. 2010). Lysine 9 of histone H3 may be mono-, di- or tri-methylated by SuvH39H1 or G9a, usually leading to transcriptional repression (Lachner et al. 2001).
Wide species cross-reactivity is expected from Rabbit anti Human tri-methyl-histone H3 (Lys9) antibody based on sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
Similar Products
Product Notes
The HIST1H3F hist1h3a (Catalog #AAA50474) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tri-Methyl-Histone H3 (Lys9) antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tri-Methyl-Histone H3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HIST1H3F hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tri-Methyl-Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.