WB (Western Blot)
(Western blot analysis of untreated HeLa cell nuclear extract (20 ug; lane 1) or Hela nuclear extract treated with colcemid (20 ug; lane 2) probed with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) at a 1 ug/ul dilution.)
Rat anti-Human Histone H3.1 Monoclonal Antibody | anti-HIST1H3F antibody
Histone H3.1 (pSer28) antibody | 5D10D4
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Histone H3.1, Antibody; Histone H3.1 (pSer28) antibody | 5D10D4; RAT ANTI HUMAN HISTONE H3.1 (pSer28); anti-HIST1H3F antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5D10D4
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Purified IgG - Liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-HIST1H3F antibody
WB (Western Blot), IF (Immunofluorescence)
Immunogen
A peptide containing phosphorylated serine 28 of human histone H3.1
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of untreated HeLa cell nuclear extract (20 ug; lane 1) or Hela nuclear extract treated with colcemid (20 ug; lane 2) probed with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) at a 1 ug/ul dilution.)
WB (Western Blot)
(Western blot analysis of untreated HeLa cell nuclear extract (20 ug; lane 1) or Hela nuclear extract treated with colcemid (20 ug; lane 2) probed with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) at a 1 ug/ul dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of formaldehyde fixed mitotic DM4 cells with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) (left), counterstained with Hoechst (right).)
IF (Immunofluorescence)
(Immunofluorescence staining of formaldehyde fixed mitotic DM4 cells with histone H3.1 (pSer28) antibody, clone 5D10D4 (AAA50437) (left), counterstained with Hoechst (right).)
Related Product Information for anti-HIST1H3F antibody
Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 recognizes Histone H3.1 when phosphorylated at serine 28.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle (Tagami et al. 2004). Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle (Zhong et al. 2003).
Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle (Tagami et al. 2004). Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle (Zhong et al. 2003).
Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
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Product Notes
The HIST1H3F hist1h3a (Catalog #AAA50437) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Histone H3.1 (pSer28) antibody | 5D10D4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the HIST1H3F hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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