Application Data
(Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A (AAA49907) following antigen retrieval using sodium citrate buffer (pH6.0))
Rabbit TRIAD3A Polyclonal Antibody | anti-TRIAD3A antibody
RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL)
Gene Names
Rnf216; UIP83; C86502; TRIAD3; AI647468; AU019462; Ubce7ip1; 2810055G22Rik; F830018F18Rik
Applications
Western Blot, Immunohistochemistry
Synonyms
TRIAD3A, Antibody; RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL); anti-TRIAD3A antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.
Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
732
Applicable Applications for anti-TRIAD3A antibody
WB (Western Blot), IHC (Immunohistochemistry)
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
A 15 amino acid peptide located near mouse TRIAD3A amino-terminus.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to mouse TRIAD3A (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A (AAA49907) following antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A (AAA49907) following antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Western blot analysis of mouse heart lysate probed with Rabbit anti Mouse TRIAD3A (AAA49907) at 0.5(A), 1(B) and 2(C) ug/ml)
Application Data
(Western blot analysis of mouse heart lysate probed with Rabbit anti Mouse TRIAD3A (AAA49907) at 0.5(A), 1(B) and 2(C) ug/ml)
Related Product Information for anti-TRIAD3A antibody
MBS221309 recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response. Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,612 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF216 isoform A
NCBI Official Synonym Full Names
ring finger protein 216
NCBI Official Symbol
Rnf216
NCBI Official Synonym Symbols
UIP83; C86502; TRIAD3; AI647468; AU019462; Ubce7ip1; 2810055G22Rik; F830018F18Rik
NCBI Protein Information
E3 ubiquitin-protein ligase RNF216; E3 ubiquitin ligase; triad domain-containing protein 3; ubcM4-interacting protein 83; ubiquitin-conjugating enzyme 7-interacting protein 1
UniProt Protein Name
E3 ubiquitin-protein ligase RNF216
UniProt Gene Name
Rnf216
UniProt Synonym Gene Names
Triad3; Ubce7ip1; Uip83; Zin
UniProt Entry Name
RN216_MOUSE
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Product Notes
The TRIAD3A rnf216 (Catalog #AAA49907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TRIAD3A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TRIAD3A rnf216 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIAD3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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