IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human TRIM28 Polyclonal Antibody | anti-TRIM28 antibody
TRIM28 Antibody (N-term)
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIM28, Antibody; TRIM28 Antibody (N-term); Transcription intermediary factor 1-beta; TIF1-beta; E3 SUMO-protein ligase TRIM28; 632-; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28; TRIM28; KAP1; RNF96; TIF1B; anti-TRIM28 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.47 (varies by lot)
Sequence Positions
178-207
Sequence Length
835
Applicable Applications for anti-TRIM28 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.)
IHC (Immunohiostchemistry)
(TRIM28 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(TRIM28 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(TRIM28 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).)
WB (Western Blot)
(TRIM28 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).)
Related Product Information for anti-TRIM28 antibody
The protein encoded by this gene mediates transcriptional
control by interaction with the Kruppel-associated box repression
domain found in many transcription factors. The protein localizes
to the nucleus and is thought to associate with specific chromatin
regions. The protein is a member of the tripartite motif family.
This tripartite motif includes three zinc-binding domains, a RING,
a B-box type 1 and a B-box type 2, and a coiled-coil region.
control by interaction with the Kruppel-associated box repression
domain found in many transcription factors. The protein localizes
to the nucleus and is thought to associate with specific chromatin
regions. The protein is a member of the tripartite motif family.
This tripartite motif includes three zinc-binding domains, a RING,
a B-box type 1 and a B-box type 2, and a coiled-coil region.
Product Categories/Family for anti-TRIM28 antibody
References
Lai, I.L., et al. J. Biol. Chem. 285(10):7187-7196(2010)
Yokoe, T., et al. Ann. Surg. Oncol. 17(3):821-828(2010)
Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010)
Li, X., et al. Sci Signal 3 (119), RA32 (2010) :
Groner, A.C., et al. PLoS Genet. 6 (3), E1000869 (2010) :
Yokoe, T., et al. Ann. Surg. Oncol. 17(3):821-828(2010)
Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010)
Li, X., et al. Sci Signal 3 (119), RA32 (2010) :
Groner, A.C., et al. PLoS Genet. 6 (3), E1000869 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88550
NCBI Official Full Name
transcription intermediary factor 1-beta
NCBI Official Synonym Full Names
tripartite motif containing 28
NCBI Official Symbol
TRIM28
NCBI Official Synonym Symbols
KAP1; TF1B; RNF96; TIF1B; PPP1R157
NCBI Protein Information
transcription intermediary factor 1-beta
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Gene Name
TRIM28
UniProt Synonym Gene Names
KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
UniProt Entry Name
TIF1B_HUMAN
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Product Notes
The TRIM28 trim28 (Catalog #AAA286333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 178-207. The TRIM28 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM28 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the TRIM28 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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