IHC (Immunohiostchemistry)
(Anti-TRPC3 antibody, AAA45179, IHC(P)IHC(P): Rat Brain Tissue)
Rabbit TRPC3 Polyclonal Antibody | anti-TRPC3 antibody
Anti-TRPC3 antibody
Gene Names
TRPC3; TRP3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
TRPC3, Antibody; Anti-TRPC3 antibody; Short transient receptor potential channel 3(TrpC3); transient receptor potential cation channel, subfamily C, member 3; Htrp 3 antibody; Htrp3 antibody; MGC124333 antibody; Mtrp3 antibody; Receptor activated cation channel TRP3 antibody; Short transient receptor potential channel 3 antibody; Transient receptor potential cation channel subfamily C member 3 antibody; Transient receptor potential channel 3 antibody; TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody; Transient receptor protein 3 antibody; Trp related protein 3 antibody; TRP3 antibody; TrpC3 antibody; Trrp3 antibody; anti-TRPC3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
836
Applicable Applications for anti-TRPC3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRPC3 (836-851aa HSFNSILNQPTRYQQI), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-TRPC3 antibody, AAA45179, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohiostchemistry)
(Anti-TRPC3 antibody, AAA45179, IHC(P)IHC(P): Rat Brain Tissue)
WB (Western Blot)
(Anti-TRPC3 antibody, AAA45179, Western blottingAll lanes: Anti TRPC3 (AAA45179) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: Rat Ovary Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: SW620 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 97KDObserved bind size: 120KD)
WB (Western Blot)
(Anti-TRPC3 antibody, AAA45179, Western blottingAll lanes: Anti TRPC3 (AAA45179) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Intestine Tissue Lysate at 50ugLane 4: Rat Ovary Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: SW620 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 97KDObserved bind size: 120KD)
Related Product Information for anti-TRPC3 antibody
Description: Rabbit IgG polyclonal antibody for Short transient receptor potential channel 3(TRPC3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
Background: TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
97,355 Da
NCBI Official Full Name
Short transient receptor potential channel 3
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily C, member 3
NCBI Official Symbol
TRPC3
NCBI Official Synonym Symbols
TRP3
NCBI Protein Information
short transient receptor potential channel 3; hTrp-3; transient receptor protein 3; transient receptor potential cation channel subfamily C member 3 variant c
UniProt Protein Name
Short transient receptor potential channel 3
UniProt Gene Name
TRPC3
UniProt Synonym Gene Names
TRP3; TrpC3; TRP-3; hTrp-3; hTrp3
UniProt Entry Name
TRPC3_HUMAN
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Product Notes
The TRPC3 trpc3 (Catalog #AAA45179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRPC3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPC3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TRPC3 trpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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