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IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.)
Rabbit TSH2 Polyclonal Antibody | anti-TSHZ2 antibody
TSH2 Antibody (N-term)
Gene Names
TSHZ2; TSH2; ZABC2; ZNF218; OVC10-2; C20orf17
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TSH2, Antibody; TSH2 Antibody (N-term); Teashirt homolog 2; Ovarian cancer-related protein 10-2; OVC10-2; Zinc finger protein 218; TSHZ2; C20orf17; TSH2; ZNF218; anti-TSHZ2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-261 amino acids from the N-terminal region of human TSH2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
232-261
Sequence Length
1,031
Applicable Applications for anti-TSHZ2 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.)
FCM/FACS (Flow Cytometry)
(TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-TSHZ2 antibody
TSH2 is transcriptional regulator involved in developmental processes (Potential).
References
Jenkins, D., et al. Nephrol. Dial. Transplant. 25(1):54-60(2010)
Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Gocke, C.B., et al. J. Biol. Chem. 280(6):5004-5012(2005)
Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Gocke, C.B., et al. J. Biol. Chem. 280(6):5004-5012(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115005
NCBI Official Full Name
teashirt homolog 2 isoform 2
NCBI Official Synonym Full Names
teashirt zinc finger homeobox 2
NCBI Official Symbol
TSHZ2
NCBI Official Synonym Symbols
TSH2; ZABC2; ZNF218; OVC10-2; C20orf17
NCBI Protein Information
teashirt homolog 2
UniProt Protein Name
Teashirt homolog 2
UniProt Gene Name
TSHZ2
UniProt Synonym Gene Names
C20orf17; TSH2; ZNF218; OVC10-2
UniProt Entry Name
TSH2_HUMAN
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Product Notes
The TSHZ2 tshz2 (Catalog #AAA284377) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 232-261. The TSH2 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's TSH2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TSHZ2 tshz2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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