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IP (Immunoprecipitation)
(Immunoprecipitating TUBA4A in K562 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA232754 in K562 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA232754(4ug)+ K562 whole cell lysate(500ug)Lane 3: K562 whole cell lysate (20ug))
Rabbit anti-Human, Mouse TUBA4A Polyclonal Antibody | anti-TUBA4A antibody
TUBA4A Antibody
Gene Names
TUBA4A; ALS22; TUBA1; H2-ALPHA
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
TUBA4A, Antibody; TUBA4A Antibody; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A; TUBA1; anti-TUBA4A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
433
Applicable Applications for anti-TUBA4A antibody
IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Tubulin alpha-4A chain protein (20-183AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31779
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating TUBA4A in K562 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA232754 in K562 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA232754(4ug)+ K562 whole cell lysate(500ug)Lane 3: K562 whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TUBA4A in K562 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA232754 in K562 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA232754(4ug)+ K562 whole cell lysate(500ug)Lane 3: K562 whole cell lysate (20ug))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232754 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232754 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using AAA232754 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using AAA232754 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA232754 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA232754 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in:NIH/3T3 whole cell lysate,K562 whole cell lysate,Mouse brain tissueAll lanes: TUBA4A antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,49 kDaObserved band size: 50,24 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:NIH/3T3 whole cell lysate,K562 whole cell lysate,Mouse brain tissueAll lanes: TUBA4A antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,49 kDaObserved band size: 50,24 kDa)
Related Product Information for anti-TUBA4A antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,329 Da
NCBI Official Full Name
tubulin alpha-4A chain isoform 2
NCBI Official Synonym Full Names
tubulin alpha 4a
NCBI Official Symbol
TUBA4A
NCBI Official Synonym Symbols
ALS22; TUBA1; H2-ALPHA
NCBI Protein Information
tubulin alpha-4A chain
UniProt Protein Name
Tubulin alpha-4A chain
UniProt Gene Name
TUBA4A
UniProt Synonym Gene Names
TUBA1
UniProt Entry Name
TBA4A_HUMAN
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Product Notes
The TUBA4A tuba4a (Catalog #AAA232754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUBA4A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TUBA4A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TUBA4A tuba4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBA4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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