IP (Immunoprecipitation)
(Immunoprecipitating TWF2 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118798 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118798(4ug)+ HepG2 whole cell lysate(500ug)Lane 3:HepG2 whole cell lysate (20ug))
Rabbit anti-Human Twinfilin-2 Polyclonal Antibody | anti-TWF2 antibody
Rabbit anti-human Twinfilin-2 polyclonal Antibody(TWF2)
Gene Names
TWF2; A6r; A6RP; PTK9L; MSTP011
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
Twinfilin-2, Antibody; Rabbit anti-human Twinfilin-2 polyclonal Antibody(TWF2); A6-related protein; hA6RP; Protein tyrosine kinase 9-like; Twinfilin-1-like protein; TWF2; PTK9L; MSTP011; anti-TWF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
349
Applicable Applications for anti-TWF2 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Twinfilin-2 protein (1-369aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-100215 / sc-100216
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating TWF2 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118798 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118798(4ug)+ HepG2 whole cell lysate(500ug)Lane 3:HepG2 whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TWF2 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118798 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118798(4ug)+ HepG2 whole cell lysate(500ug)Lane 3:HepG2 whole cell lysate (20ug))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA118798 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA118798 at dilution 1:100)
WB (Western Blot)
(All lanes: Twinfilin-2 antibody at 3ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateLane 3:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
WB (Western Blot)
(All lanes: Twinfilin-2 antibody at 3ug/mlLane 1:Jurkat whole cell lysateLane 2:HepG2 whole cell lysateLane 3:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Related Product Information for anti-TWF2 antibody
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,548 Da
NCBI Official Full Name
twinfilin-2
NCBI Official Synonym Full Names
twinfilin actin binding protein 2
NCBI Official Symbol
TWF2
NCBI Official Synonym Symbols
A6r; A6RP; PTK9L; MSTP011
NCBI Protein Information
twinfilin-2
UniProt Protein Name
Twinfilin-2
UniProt Gene Name
TWF2
UniProt Synonym Gene Names
PTK9L; hA6RP
UniProt Entry Name
TWF2_HUMAN
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Product Notes
The TWF2 twf2 (Catalog #AAA118798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Twinfilin-2 polyclonal Antibody(TWF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Twinfilin-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TWF2 twf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Twinfilin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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