Rabbit anti-Human Tyrosine-protein kinase Lck Polyclonal Antibody | anti-Lck antibody

Rabbit anti-human Tyrosine-protein kinase Lck polyclonal Antibody

Cat. Num. AAA119688

• Gene Names: LCK; LSK; YT16; IMD22; p56lck; pp58lck
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Tyrosine-protein kinase Lck, Antibody; Rabbit anti-human Tyrosine-protein kinase Lck polyclonal Antibody; Leukocyte C-terminal Src kinase; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; LCK; anti-Lck antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 509

• Applicable Applications for anti-Lck antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Tyrosine-protein kinase Lck protein(1-539aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-32014 / sc-13 / sc-28882
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating LCK in Jurkat whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA119688 in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA119688 (8ug)+ Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))

IHC (Immunohistochemisry)

(Immunofluorescent analysis of Hela cells using AAA119688 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using AAA119688 at dilution of 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA119688 at dilution of 1:100)

Related Product Information for anti-Lck antibody

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.

NCBI and Uniprot Product Information

• NCBI GI #: 112789548
• NCBI GeneID: 3932
• NCBI Accession #: NP_001036236.1
• NCBI GenBank Nucleotide #: NM_001042771.2
• UniProt Accession #: P06239; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8
• Molecular Weight: 61,190 Da
• NCBI Official Full Name: tyrosine-protein kinase Lck
• NCBI Official Synonym Full Names: LCK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; IMD22; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck
• UniProt Protein Name: Tyrosine-protein kinase Lck
• UniProt Gene Name: LCK
• UniProt Synonym Gene Names: LSK
• UniProt Entry Name: LCK_HUMAN