IP (Immunoprecipitation)
(Detection of human UACA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UACA antibody AAA211449 used for IP at 3 ug/mg lysate. UACA was also immunoprecipitated by rabbit anti-UACA antibodies and which recognize downstream epitopes. For blotting immunoprecipitated UACA, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human UACA Polyclonal Antibody | anti-UACA antibody
Rabbit anti-UACA Antibody, Affinity Purified
Gene Names
UACA; NUCLING
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
UACA, Antibody; Rabbit anti-UACA Antibody, Affinity Purified; NUCLING; uveal autoantigen with coiled-coil domains and ankyrin repeats; nuclear membrane binding protein; Uveal autoantigen with coiled-coil domains and ankyrin repeats; anti-UACA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-UACA antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human UACA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UACA antibody AAA211449 used for IP at 3 ug/mg lysate. UACA was also immunoprecipitated by rabbit anti-UACA antibodies and which recognize downstream epitopes. For blotting immunoprecipitated UACA, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human UACA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UACA antibody AAA211449 used for IP at 3 ug/mg lysate. UACA was also immunoprecipitated by rabbit anti-UACA antibodies and which recognize downstream epitopes. For blotting immunoprecipitated UACA, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human UACA by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-UACA (Cat. No. AAA211449 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human UACA by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-UACA (Cat. No. AAA211449 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-UACA antibody
UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.
Product Categories/Family for anti-UACA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
161,510 Da
NCBI Official Full Name
uveal autoantigen with coiled-coil domains and ankyrin repeats isoform 1
NCBI Official Synonym Full Names
uveal autoantigen with coiled-coil domains and ankyrin repeats
NCBI Official Symbol
UACA
NCBI Official Synonym Symbols
NUCLING
NCBI Protein Information
uveal autoantigen with coiled-coil domains and ankyrin repeats; nuclear membrane binding protein; uveal autoantigen with coiled coil domains and ankyrin repeats
UniProt Protein Name
Uveal autoantigen with coiled-coil domains and ankyrin repeats
UniProt Gene Name
UACA
UniProt Synonym Gene Names
KIAA1561
UniProt Entry Name
UACA_HUMAN
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Product Notes
The UACA uaca (Catalog #AAA211449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UACA Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UACA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the UACA uaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UACA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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