Rabbit UBA52 Polyclonal Antibody | anti-UBA52 antibody

Phospho-UBA52 (Ser65) Antibody

Cat. Num. AAA329353

• Gene Names: UBA52; L40; CEP52; RPL40; HUBCEP52
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: UBA52, Antibody; Phospho-UBA52 (Ser65) Antibody; 60S ribosomal protein L40; CEP52; HUBCEP52; L40; MGC126879; MGC57125; RPL40; UBA 52; Ubiquitin 52 amino acid fusion protein; Ubiquitin 60S ribosomal protein L40; Ubiquitin A 52 residue ribosomal protein fusion product 1; Ubiquitin carboxyl extension protein 52; Ubiquitin CEP52; anti-UBA52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-UBA52 (Ser65) Antibody detects endogenous levels of UBA52 only when phosphorylated at Ser65.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-UBA52 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human UBA52 around the phosphorylation site of Ser65.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
• Subunit Structure: Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).
• Similarity: In the N-terminal section; belongs to the ubiquitin family.In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.
• Subcellular Location: Cytoplasm. Nucleus. Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from P19 cells(lps treatment), using Phospho-UBA52 (Ser65) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 11kDa)

Related Product Information for anti-UBA52 antibody

Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 77539055
• NCBI GeneID: 7311
• NCBI Accession #: NP_001029102.1
• NCBI GenBank Nucleotide #: NM_001033930.1
• UniProt Accession #: P62987; P02248; P02249; P02250; P14793; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888
• Molecular Weight: 14,728 Da
• NCBI Official Full Name: ubiquitin-60S ribosomal protein L40
• NCBI Official Synonym Full Names: ubiquitin A-52 residue ribosomal protein fusion product 1
• NCBI Official Symbol: UBA52
• NCBI Official Synonym Symbols: L40; CEP52; RPL40; HUBCEP52
• NCBI Protein Information: ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-52 amino acid fusion protein; ubiquitin carboxyl extension protein 52
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40
• UniProt Gene Name: UBA52
• UniProt Synonym Gene Names: UBCEP2
• UniProt Entry Name: RL40_HUMAN