WB (Western Blot)
(Western blot analysis of lysate from Hela cell line, using UBE2D2 Antibody (C-term). AAA285940 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit UBE2D2 Polyclonal Antibody | anti-UBE2D2 antibody
UBE2D2 Antibody (C-term)
Gene Names
UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus)
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
UBE2D2, Antibody; UBE2D2 Antibody (C-term); Ubiquitin-conjugating enzyme E2 D2; Ubiquitin carrier protein D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-protein ligase D2; p53-regulated ubiquitin-conjugating enzyme 1; UBE2D2; PUBC1; UBC4; UBC5B; UBCH4; UBCH5B; anti-UBE2D2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
IgG
Specificity
This UBE2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human UBE2D2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
112-140
Sequence Length
147
Applicable Applications for anti-UBE2D2 antibody
ELISA, WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from Hela cell line, using UBE2D2 Antibody (C-term). AAA285940 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from Hela cell line, using UBE2D2 Antibody (C-term). AAA285940 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-UBE2D2 antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Product Categories/Family for anti-UBE2D2 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wu, K., et al. Mol. Cell 37(6):784-796(2010)
Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010)
Sakata, E., et al. Structure 18(1):138-147(2010)
Kamadurai, H.B., et al. Mol. Cell 36(6):1095-1102(2009)
Wu, K., et al. Mol. Cell 37(6):784-796(2010)
Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010)
Sakata, E., et al. Structure 18(1):138-147(2010)
Kamadurai, H.B., et al. Mol. Cell 36(6):1095-1102(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D2 isoform 1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2D 2
NCBI Official Symbol
UBE2D2
NCBI Official Synonym Symbols
UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D2
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D2
UniProt Gene Name
UBE2D2
UniProt Synonym Gene Names
PUBC1; UBC4; UBC5B; UBCH4; UBCH5B
UniProt Entry Name
UB2D2_HUMAN
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Product Notes
The UBE2D2 ube2d2 (Catalog #AAA285940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 112-140. The UBE2D2 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2D2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UBE2D2 ube2d2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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