Rabbit anti-Human, rat UBE2N Polyclonal Antibody | anti-UBE2N antibody

UBE2N Antibody (Center)

Cat. Num. AAA286917

• Gene Names: UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• Reactivity: Human, rat
• Applications: ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: UBE2N, Antibody; UBE2N Antibody (Center); Ubiquitin-conjugating enzyme E2 N; Bendless-like ubiquitin-conjugating enzyme; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N; UBE2N; BLU; anti-UBE2N antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This UBE2N antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 41-74 amino acids from the Central region of human UBE2N.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.46 (varies by lot)
• Sequence Length: 152

• Applicable Applications for anti-UBE2N antibody: ELISA, WB (Western Blot)
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-UBE2N Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size :17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-UBE2N Antibody (Center) at 1:500 dilution + C6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-UBE2N Antibody (Center) at 1:1000 dilutionLane 1: HL-60 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-UBE2N antibody

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).

References

Yamaguchi T.,et al.J. Biochem. 120:494-497(1996).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Zou W.,et al.Biochem. Biophys. Res. Commun. 336:61-68(2005).
Hofmann R.M.,et al.Cell 96:645-653(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4507793
• NCBI GeneID: 7334
• NCBI Accession #: NP_003339.1
• NCBI GenBank Nucleotide #: NM_003348.3
• UniProt Accession #: P61088; Q16781; Q53Y81
• Molecular Weight: 17138
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 N
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2N
• NCBI Official Symbol: UBE2N
• NCBI Official Synonym Symbols: UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 N
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 N
• UniProt Gene Name: UBE2N
• UniProt Synonym Gene Names: BLU
• UniProt Entry Name: UBE2N_HUMAN