WB (Western Blot)
(Detection of human and mouse UBE4B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, U2OS, K-562, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody AAA211325 (lot AAA211325-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse UBE4B Polyclonal Antibody | anti-UBE4B antibody
Rabbit anti-UBE4B Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
UBE4B, Antibody; Rabbit anti-UBE4B Antibody, Affinity Purified; Ubiquitin fusion degradation protein 2; UFD2A; ubiquitin-fusion degradation protein 2; UBOX3; UFD2A-III/UBE4B-III splice isoform; ubiquitination factor E4B (UFD2 homolog, yeast); RING-type E3 ubiquitin transferase E4 B; homozygously deleted in neuroblastoma-1; Homozygously deleted in neuroblastoma 1; homologous to yeast UFD2; HDNB1; E4; UFD2; ubiquitin conjugation factor E4 B; Ubiquitin conjugation factor E4 B; ubiquitination factor E4B; anti-UBE4B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-UBE4B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse UBE4B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, U2OS, K-562, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody AAA211325 (lot AAA211325-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse UBE4B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, U2OS, K-562, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody AAA211325 (lot AAA211325-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human UBE4B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE4B antibody AAA211325 (lot AAA211325-3) used for IP at 6 ug per reaction. UBE4B was also immunoprecipitated by a previous lot of this antibody (lot AAA211325-2) and rabbit anti-UBE4B antibody For blotting immunoprecipitated UBE4B, AAA211325 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human UBE4B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE4B antibody AAA211325 (lot AAA211325-3) used for IP at 6 ug per reaction. UBE4B was also immunoprecipitated by a previous lot of this antibody (lot AAA211325-2) and rabbit anti-UBE4B antibody For blotting immunoprecipitated UBE4B, AAA211325 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-UBE4B antibody
Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.
Product Categories/Family for anti-UBE4B antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The UBE4B ube4b (Catalog #AAA211325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UBE4B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE4B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UBE4B ube4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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