IHC (Immunohistochemisry)
(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)
Rabbit Ubiquitin Polyclonal Antibody | anti-UBB antibody
Ubiquitin Antibody: ATTO 390
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
Applications
Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Synonyms
Ubiquitin, Antibody; Ubiquitin Antibody: ATTO 390; Polyubiquitin B; RPS27A; UBA52; UBB; UBC; ubiquitin B; anti-UBB antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
Clonality
Polyclonal
Form/Format
Protein A Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
305
Applicable Applications for anti-UBB antibody
IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
IHC (Immunohistochemisry)
(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
WB (Western Blot)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)
Related Product Information for anti-UBB antibody
Background Info: The antibody recognizes ~10kDa corresponding to free ubiquitin of human, mouse, monkey, bovine and rat origins. It also recognizes ubiquinated proteins.
Scientific Background: Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic
Scientific Background: Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic
Product Categories/Family for anti-UBB antibody
References
1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 3. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57. 4. Boston Biochem: "Ubiquitin Proteasome Pathway Overview" http://www.bostonbiochem.com/upp.php 5. Yang M., et al. (1998) J Exp Med. 187: 1835-1846. 6. Chen Z.J., et al. (1996) Cell 84: 853-862.
Anne Bailey and Lorne J. Hofseth. A method to enhance the sensitivity and reproducibility of immunohistochemistry http://www.prohisto.com/pdfs/Hofseth_Manuscript-81.pdf
Anne Bailey and Lorne J. Hofseth. A method to enhance the sensitivity and reproducibility of immunohistochemistry http://www.prohisto.com/pdfs/Hofseth_Manuscript-81.pdf
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,308 Da
NCBI Official Full Name
polyubiquitin-B
NCBI Official Synonym Full Names
ubiquitin B<
NCBI Official Symbol
UBB
NCBI Protein Information
polyubiquitin-B
UniProt Protein Name
Polyubiquitin-B
UniProt Gene Name
UBB
UniProt Entry Name
UBB_BOVIN
Similar Products
Product Notes
The UBB ubb (Catalog #AAA103611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Antibody: ATTO 390 reacts with Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout) and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UBB ubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.