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IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human UHRF1 Polyclonal Antibody | anti-UHRF1 antibody
UHRF1 Polyclonal Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purification
Synonyms
UHRF1; Polyclonal Antibody; UHRF1 Polyclonal Antibody; FLJ21925; ICBP90; MGC138707; Np95; RNF106; hNP95; anti-UHRF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
793
Applicable Applications for anti-UHRF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
IP: 1:20 - 1:50
CHIP: 1:20 - 1:50
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
IP: 1:20 - 1:50
CHIP: 1:20 - 1:50
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human UHRF1
Calculated Molecular Weight
91kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using UHRF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using UHRF1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using UHRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using UHRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-UHRF1 antibody
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.
Product Categories/Family for anti-UHRF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
793
NCBI Official Full Name
E3 ubiquitin-protein ligase UHRF1
NCBI Official Synonym Full Names
ubiquitin-like with PHD and ring finger domains 1
NCBI Official Symbol
UHRF1
NCBI Official Synonym Symbols
Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95
NCBI Protein Information
E3 ubiquitin-protein ligase UHRF1; nuclear protein 95; RING finger protein 106; nuclear phosphoprotein 95; transcription factor ICBP90; nuclear zinc finger protein Np95; inverted CCAAT box-binding protein of 90 kDa; ubiquitin-like PHD and RING finger domain-containing protein 1
UniProt Protein Name
E3 ubiquitin-protein ligase UHRF1
UniProt Gene Name
UHRF1
UniProt Synonym Gene Names
ICBP90; NP95; RNF106; HuNp95; hNp95; hUHRF1
UniProt Entry Name
UHRF1_HUMAN
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Product Notes
The UHRF1 uhrf1 (Catalog #AAA10713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UHRF1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UHRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP. WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:20 - 1:50 IP: 1:20 - 1:50 CHIP: 1:20 - 1:50. Researchers should empirically determine the suitability of the UHRF1 uhrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UHRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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