Rabbit USP13 Polyclonal Antibody | anti-USP13 antibody

USP13 Antibody (Center)

Cat. Num. AAA288529

• Gene Names: USP13; ISOT3; IsoT-3
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: USP13, Antibody; USP13 Antibody (Center); Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13; USP13; ISOT3; anti-USP13 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This USP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the Central region of human USP13.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 389-419
• Sequence Length: 863

• Applicable Applications for anti-USP13 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 Pab. (Data provided by Amy Chen, Burnham Institute for Medical Research))

WB (Western Blot)

(The anti-USP13 Pab is used in Western blot to detect USP13 in HeLa cell lysate.)

WB (Western Blot)

(Anti-USP13 Antibody (V404)at 1:2000 dilution + human testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 97. 3 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-USP13 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP13 antibody

Cell Biology

References

Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).
Timms, K.M., et al., Gene 217 (1-2), 101-106 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 215598688
• NCBI GeneID: 8975
• NCBI Accession #: NP_003931.2
• NCBI GenBank Nucleotide #: NM_003940.2
• UniProt Accession #: Q92995; Q96B25; A8K2S3; B4DYF3; D3DNS2
• Molecular Weight: 97327
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 13
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 13 (isopeptidase T-3)
• NCBI Official Symbol: USP13
• NCBI Official Synonym Symbols: ISOT3; IsoT-3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 13
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 13
• UniProt Gene Name: USP13
• UniProt Synonym Gene Names: ISOT3; ISOT-3
• UniProt Entry Name: UBP13_HUMAN