IP (Immunoprecipitation)
(USP13 was immunoprecipitated using:Lane A:0.5 mg A375 Whole Cell Lysate4 uL anti-USP13 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-USP13 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size :116 kDa)
Rabbit anti-Human USP13 Polyclonal Antibody | anti-USP13 antibody
Anti-USP13 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
USP13, Antibody; Anti-USP13 Antibody, Rabbit Polyclonal; USP13 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-IsoT-3 Antibody; Anti-ISOT3 Antibody; anti-USP13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human USP13
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-USP13 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human USP13 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human USP13 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(USP13 was immunoprecipitated using:Lane A:0.5 mg A375 Whole Cell Lysate4 uL anti-USP13 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-USP13 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size :116 kDa)
IP (Immunoprecipitation)
(USP13 was immunoprecipitated using:Lane A:0.5 mg A375 Whole Cell Lysate4 uL anti-USP13 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-USP13 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size :116 kDa)
WB (Western Blot)
(Anti-USP13 rabbit polyclonal antibody at 1:500 dilutionLane A: A375 Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:97 kDaObserved band size:116 kDa)
WB (Western Blot)
(Anti-USP13 rabbit polyclonal antibody at 1:500 dilutionLane A: A375 Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:97 kDaObserved band size:116 kDa)
Related Product Information for anti-USP13 antibody
Deubiquitination is a reverse process of cellular ubiquitination important for many biological events. Ubiquitin (Ub)-specific protease 13 (USP13) is an ortholog of USP5 implicated in catalyzing hydrolysis of various Ub chains. USP13 is a de-ubiquinating enzyme that regulates protein ubiquitination and clearance. USP13 specifically deubiquitinates and thus upregulates ATP citrate lyase and oxoglutarate dehydrogenase, two key enzymes that determine mitochondrial respiration, glutaminolysis and fatty acid synthesis. Ubiquitin-specific peptidase 13 (USP13) is a master regulator that drives ovarian cancer metabolism. USP13 may be a potential therapeutic target for the treatment of various malignancies.
References
Zhang YH, et al. (2011) Domain analysis reveals that a deubiquitinating enzyme usp13 performs non-activating catalysis for lys63-linked polyubiquitin. PLoS One 6 (12): e29362.Xiang S, et al. (2015) Microrna-135b regulates the stability of pten and promotes glycolysis by targeting usp13 in human colorectal cancers. Oncol Rep 33 (3): 1342-1348.Han C, et al. (2016) Amplification of usp13 drives ovarian cancer metabolism. Nat Commun 7 13525.
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Product Notes
The USP13 (Catalog #AAA256694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-USP13 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP13 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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