IF (Immunofluorescence)
(Immunofluorescence staining of USP38 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human USP38 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Rabbit anti-Human USP38 Polyclonal Antibody | anti-USP38 antibody
Anti-USP38 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
USP38, Antibody; Anti-USP38 Antibody, Rabbit Polyclonal; USP38 Antibody, Rabbit PAb, Antigen Affinity Purified; Ubiquitin specific peptidase 38; Anti-HP43.8KD Antibody; anti-USP38 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human USP38
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-USP38 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human USP38 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human USP38 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of USP38 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human USP38 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of USP38 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human USP38 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
WB (Western Blot)
(Anti-USP38 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:117 kDa)
WB (Western Blot)
(Anti-USP38 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:117 kDa)
Related Product Information for anti-USP38 antibody
USP38 (Ubiquitin Specific Peptidase 38) is a Protein Coding gene. The USP38 gene, located on 4q31.21, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. USP38 belongs to the peptidase C19 family. 2 alternatively spliced human isoforms have been reported. USP38 is an LSD1-specific deubiquitinase that affects cellular physiology by interacting with LSD1. USP38 represents the first identified deubiquitinase specifically for Th2 immunity and the associated asthma. It is widely expressed in bone marrow, colon, and other tissues. Diseases associated with USP38 include Ullrich Congenital Muscular Dystrophy 1. 241 organisms have orthologs with human gene USP38.
References
Yang Y, et al. (2020) The deubiquitinase usp38 promotes nhej repair through regulation of hdac1 activity and regulates cancer cell response to genotoxic insults. Cancer Res 80 (4): 719-731.Liu W, et al. (2018) The deubiquitinase usp38 affects cellular functions through interacting with lsd1. Biol Res 51 (1): 53.Chen S, et al. (2018) Usp38 critically promotes asthmatic pathogenesis by stabilizing junb protein. J Exp Med 215 (11): 2850-2867.
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Product Notes
The USP38 (Catalog #AAA256042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-USP38 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP38 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the USP38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP38, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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