WB (Western Blot)
(Detection of human USP4 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP4 antibody (AAA211170 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human USP4 Polyclonal Antibody | anti-USP4 antibody
Rabbit anti-USP4 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP4, Antibody; Rabbit anti-USP4 Antibody, Affinity Purified; ubiquitin thiolesterase 4; Unph; UNP; ubiquitous nuclear protein homolog; ubiquitin-specific processing protease 4; ubiquitin thioesterase 4; ubiquitin specific protease 4 (proto-oncogene); ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin carboxyl-terminal hydrolase 4; ubiquitin carboxyl-terminal esterase 4; deubiquitinating enzyme 4; Ubiquitin-specific-processing protease 4; Ubiquitin carboxyl-terminal hydrolase 4; ubiquitin specific peptidase 4; anti-USP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-USP4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Dog, Horse, Rabbit, Pig, Panda, Orangutan, Monkey, Chimpanzee
Immunogen
Between 913 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human USP4 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP4 antibody (AAA211170 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human USP4 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP4 antibody (AAA211170 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody (AAA211170 lot 3) used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by a previous lot of this antibody (AAA211170 lot 2) and a second antibody against a different epitope of USP4 . For blotting immunoprecipitated USP4, AAA211170 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody (AAA211170 lot 3) used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by a previous lot of this antibody (AAA211170 lot 2) and a second antibody against a different epitope of USP4 . For blotting immunoprecipitated USP4, AAA211170 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-USP4 antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 4 (USP4) is a member of this family.
Product Categories/Family for anti-USP4 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP4 usp4 (Catalog #AAA211170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP4 usp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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