WB (Western Blot)
(Detection of human USP8 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP8 antibody AAA212075 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human USP8 Polyclonal Antibody | anti-USP8 antibody
Rabbit anti-USP8 Antibody, Affinity Purified
Gene Names
USP8; UBPY; HumORF8; FLJ34456; KIAA0055; MGC129718
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP8, Antibody; Rabbit anti-USP8 Antibody, Affinity Purified; ubiquitin isopeptidase Y; UBPY; ubiquitin-specific-processing protease 8; Ubiquitin thioesterase 8; ubiquitin carboxyl-terminal hydrolase 8; SPG59; PITA4; HumORF8; hUBPy; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; Ubiquitin carboxyl-terminal hydrolase 8; ubiquitin specific peptidase 8; anti-USP8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-USP8 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human USP8 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP8 antibody AAA212075 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human USP8 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP8 antibody AAA212075 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human USP8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP8 (Cat. No. AAA212075 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human USP8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP8 (Cat. No. AAA212075 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-USP8 antibody
USP8 (ubiquitin-specific processing protease 8) is also known as UBPY (ubiquitin isopeptidase Y). USP8 is a hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P40818].
Product Categories/Family for anti-USP8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
127,523 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 8
NCBI Official Synonym Full Names
ubiquitin specific peptidase 8
NCBI Official Symbol
USP8
NCBI Official Synonym Symbols
UBPY; HumORF8; FLJ34456; KIAA0055; MGC129718
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 8; hUBPy; OTTHUMP00000162416; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; ubiquitin specific protease 8; ubiquitin-specific-processing protease 8
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 8
UniProt Gene Name
USP8
UniProt Synonym Gene Names
KIAA0055; UBPY; hUBPy
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Product Notes
The USP8 usp8 (Catalog #AAA212075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP8 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP8 usp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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