SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
USP8 recombinant protein
USP8, Active
Applications
Western Blot
Synonyms
USP8; N/A; USP8, Active; KIAA0055; UBPY; USP8 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for USP8 recombinant protein
WB (Western Blot), Activity Assay
Tag
HIS
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for USP8 recombinant protein
Recombinant human USP8 (630-end) was expressed in E Coli cells using an N-terminal His tag. The USP8 gene accession number is NM_005154.
USP8 (ubiquitin carboxyl-terminal hydrolase 8) is a member of the ubiquitin-specific processing protease family of proteins and is involved in the regulation of endosomal ubiquitin processes for endosome morphology, cargo sorting and membrane trafficking at the early endosome stage. USP8 deubiquitinates EPS15 and regulates EGFR degradation and MAPK signaling via removing conjugated ubiquitin from EGFR (1). USP8 also interacts with spermatid ESCRT-0 and microtubule structures in mouse species, suggesting that it may be involved in acrosome biogenesis (2).
USP8 (ubiquitin carboxyl-terminal hydrolase 8) is a member of the ubiquitin-specific processing protease family of proteins and is involved in the regulation of endosomal ubiquitin processes for endosome morphology, cargo sorting and membrane trafficking at the early endosome stage. USP8 deubiquitinates EPS15 and regulates EGFR degradation and MAPK signaling via removing conjugated ubiquitin from EGFR (1). USP8 also interacts with spermatid ESCRT-0 and microtubule structures in mouse species, suggesting that it may be involved in acrosome biogenesis (2).
Product Categories/Family for USP8 recombinant protein
References
1. Berlin, I. et al: Regulation of epidermal growth factor receptor ubiquitination and trafficking by the USP8-STAM complex. Journal of biological chemistry 285(45), 34909-21, 2010.
2. Giovanna,B. et al. USP8, a Regulator of Endosomal Sorting, Is Involved in Mouse Acrosome Biogenesis Through Interaction with the Spermatid ESCRT-0 Complex and Microtubules, Biology of Reproduction, 82(5), 930-939, 2010.
2. Giovanna,B. et al. USP8, a Regulator of Endosomal Sorting, Is Involved in Mouse Acrosome Biogenesis Through Interaction with the Spermatid ESCRT-0 Complex and Microtubules, Biology of Reproduction, 82(5), 930-939, 2010.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP8 (Catalog #AAA74098) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's USP8 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Activity Assay. Researchers should empirically determine the suitability of the USP8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.