WB (Western Blot)
(Detection of human and mouse VAP-33 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody AAA212648 (lot AAA212648-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse VAP-33 Polyclonal Antibody | anti-VAP-33 antibody
Rabbit anti-VAP-33 Antibody, Affinity Purified
Gene Names
VAPA; VAP-A; VAP33; VAP-33; hVAP-33
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
VAP-33, Antibody; Rabbit anti-VAP-33 Antibody, Affinity Purified; VAMP-A; VAP-A; VAP-33; vesicle-associated membrane protein-associated protein A; VAMP-associated protein A; hVAP-33; epididymis secretory sperm binding protein; 33 kDa VAMP-associated protein; VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa; VAP33; Vesicle-associated membrane protein-associated protein A; VAMP associated protein A; anti-VAP-33 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-VAP-33 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse VAP-33 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody AAA212648 (lot AAA212648-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse VAP-33 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody AAA212648 (lot AAA212648-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human VAP-33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody AAA212648 (lot AAA212648-1) used for IP at 6 ug per reaction. VAP-33 was also immunoprecipitated by rabbit anti-VAP-33 antibody BL16444. For blotting immunoprecipitated VAP-33, AAA212648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human VAP-33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody AAA212648 (lot AAA212648-1) used for IP at 6 ug per reaction. VAP-33 was also immunoprecipitated by rabbit anti-VAP-33 antibody BL16444. For blotting immunoprecipitated VAP-33, AAA212648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human VAP-33 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-VAP-33 (Cat. No. AAA212648 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human VAP-33 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-VAP-33 (Cat. No. AAA212648 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-VAP-33 antibody
VAP-33 is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. VAP-33 may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility [taken from NCBI Entrez Gene (Gene ID: 9218)].
Product Categories/Family for anti-VAP-33 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,614 Da
NCBI Official Full Name
vesicle-associated membrane protein-associated protein A isoform 2
NCBI Official Synonym Full Names
VAMP associated protein A
NCBI Official Symbol
VAPA
NCBI Official Synonym Symbols
VAP-A; VAP33; VAP-33; hVAP-33
NCBI Protein Information
vesicle-associated membrane protein-associated protein A
UniProt Protein Name
Vesicle-associated membrane protein-associated protein A
UniProt Gene Name
VAPA
UniProt Synonym Gene Names
VAP33; VAMP-A; VAMP-associated protein A; VAP-A; VAP-33
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Product Notes
The VAP-33 vapa (Catalog #AAA212648) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-VAP-33 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VAP-33 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the VAP-33 vapa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAP-33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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