IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of A431 cells treated by Forskolin using 2.5ug Phospho-VASP-S157 antibody. Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 antibody at a dilition of 1:1000.)
Rabbit anti-Human, Rat VASP-S157 Polyclonal Antibody | anti-VASP-S157 antibody
Phospho-VASP-S157 pAb
Reactivity
Human, Rat
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purification
Synonyms
VASP-S157, Antibody; Phospho-VASP-S157 pAb; anti-VASP-S157 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence
RVSNA
Sequence Length
380
Applicable Applications for anti-VASP-S157 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding S157 of human VASP
Immunogen Species
Human
Cellular Location
Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of A431 cells treated by Forskolin using 2.5ug Phospho-VASP-S157 antibody. Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of A431 cells treated by Forskolin using 2.5ug Phospho-VASP-S157 antibody. Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 antibody at a dilition of 1:1000.)
WB (Western Blot)
(Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-VASP-S157 antibody
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Product Categories/Family for anti-VASP-S157 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 39kDa
Observed: 50kDa
Observed: 50kDa
NCBI Official Full Name
vasodilator-stimulated phosphoprotein
NCBI Official Synonym Full Names
vasodilator stimulated phosphoprotein
NCBI Official Symbol
VASP
NCBI Protein Information
vasodilator-stimulated phosphoprotein
UniProt Protein Name
Vasodilator-stimulated phosphoprotein
UniProt Gene Name
VASP
UniProt Synonym Gene Names
VASP
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Product Notes
The VASP-S157 vasp (Catalog #AAA281282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-VASP-S157 pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VASP-S157 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the VASP-S157 vasp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RVSNA. It is sometimes possible for the material contained within the vial of "VASP-S157, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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