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WB (Western Blot)
(Detection of human VCP by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, GaMG, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse VCP Polyclonal Antibody | anti-VCP antibody
Rabbit anti-VCP Antibody, Affinity Purified
Gene Names
VCP; p97; TERA; ALS14; IBMPFD
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
VCP, Antibody; Rabbit anti-VCP Antibody, Affinity Purified; p97; VCP; Valosin-containing protein; transitional endoplasmic reticulum ATPase; CDC48; 15S Mg(2+)-ATPase p97 subunit; TERA; TER ATPase; Transitional endoplasmic reticulum ATPase; valosin containing protein; anti-VCP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-VCP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Pig
Immunogen
Between 756 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human VCP by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, GaMG, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human VCP by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, GaMG, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse VCP by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse VCP by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for IP at 6 ug per reaction. VCP was also immunoprecipitated by a previous lot of this antibody (AAA211038 lot 3). For blotting immunoprecipitated VCP, AAA211038 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody (AAA211038 lot 4) used for IP at 6 ug per reaction. VCP was also immunoprecipitated by a previous lot of this antibody (AAA211038 lot 3). For blotting immunoprecipitated VCP, AAA211038 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of human VCP by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti- VCP (Cat. No. AAA211038 Lot4) used at a dilution of 1:2000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human VCP by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti- VCP (Cat. No. AAA211038 Lot4) used at a dilution of 1:2000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse VCP by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- VCP (Cat. No. AAA211038 Lot4) used at a dilution of 1:2000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse VCP by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- VCP (Cat. No. AAA211038 Lot4) used at a dilution of 1:2000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-VCP antibody
Valosin-containing protein (VCP) is a multifunctional protein that is proposed to be involved in vesicle transport and fusion for protein trafficking, 26S proteasome function in ubiquitin-based degradation pathways, and the assembly of peroxisomes. Mutations in the human VCP gene have been associated with diseases such as inclusion body myopathy, Paget disease of the bone, and frontotemporal dementia (IBMPFD). VCP can inhibit apoptosis and may play a role in tumorigenesis.
Product Categories/Family for anti-VCP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,322 Da
NCBI Official Full Name
transitional endoplasmic reticulum ATPase
NCBI Official Synonym Full Names
valosin containing protein
NCBI Official Symbol
VCP
NCBI Official Synonym Symbols
p97; TERA; ALS14; IBMPFD
NCBI Protein Information
transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit
UniProt Protein Name
Transitional endoplasmic reticulum ATPase
UniProt Gene Name
VCP
UniProt Synonym Gene Names
TER ATPase; VCP
UniProt Entry Name
TERA_HUMAN
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Product Notes
The VCP vcp (Catalog #AAA211038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-VCP Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VCP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the VCP vcp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VCP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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