IHC (Immunohiostchemistry)
(Anti- Versican Picoband antibody, AAA46532, IHC(P)IHC(P): Human Glioma Tissue)
Versican Polyclonal Antibody | anti-VCAN antibody
Anti-Versican Antibody
Gene Names
VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Versican, Antibody; Anti-Versican Antibody; Versican core protein; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2_HUMAN; ERVR; GHAP; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PGM; VCAN; Versican proteoglycan; WGN 1; WGN; WGN1 antibody; versican; anti-VCAN antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
655
Applicable Applications for anti-VCAN antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti- Versican Picoband antibody, AAA46532, IHC(P)IHC(P): Human Glioma Tissue)
IHC (Immunohiostchemistry)
(Anti- Versican Picoband antibody, AAA46532, IHC(P)IHC(P): Human Glioma Tissue)
WB (Western Blot)
(Anti- Versican Picoband antibody, AAA46532, Western blottingAll lanes: Anti Versican (AAA46532) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 373KDObserved bind size: 373KD)
WB (Western Blot)
(Anti- Versican Picoband antibody, AAA46532, Western blottingAll lanes: Anti Versican (AAA46532) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 373KDObserved bind size: 373KD)
Related Product Information for anti-VCAN antibody
Description: Rabbit IgG polyclonal antibody for Versican core protein(VCAN) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
References
1. Bode-Lesniewska, B., Dours-Zimmermann, M. T., Odermatt, B. F., Briner, J., Heitz, P. U., Zimmermann, D. R. Distribution of the large aggregating proteoglycan versican in adult human tissues. J. Histochem. Cytochem. 44: 303-312, 1996. 2. Dours-Zimmermann, M. T., Zimmermann, D. R. A novel glycosaminoglycan attachment domain identified in two alternative splice variants of human versican. J. Biol. Chem. 269: 32992-32998, 1994. 3. Naso, M. F., Morgan, J. L., Buchberg, A. M., Siracusa, L. D., Iozzo, R. V. Expression pattern and mapping of the murine versican gene (Cspg2) to chromosome 13. Genomics 29: 297-300, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
369,688 Da
NCBI Official Full Name
versican core protein isoform 2
NCBI Official Synonym Full Names
versican
NCBI Official Symbol
VCAN
NCBI Official Synonym Symbols
WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
NCBI Protein Information
versican core protein
UniProt Protein Name
Versican core protein
UniProt Gene Name
VCAN
UniProt Synonym Gene Names
CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
UniProt Entry Name
CSPG2_HUMAN
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Product Notes
The VCAN vcan (Catalog #AAA46532) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Versican Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Versican can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the VCAN vcan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Versican, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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