IF (Immunofluorescence)
(Immunofluorescence analysis of U-251MG cells using VHL Polyclonal Antibody (AAA10644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
VHL Polyclonal Antibody | anti-VHL antibody
VHL Polyclonal Antibody
Gene Names
VHL; RCA1; VHL1; pVHL; HRCA1
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purification
Synonyms
VHL, Antibody; VHL Polyclonal Antibody; VHL; HRCA1; RCA1; VHL1; pVHL; von Hippel-Lindau tumor suppressor; anti-VHL antibody
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence Length
213
Applicable Applications for anti-VHL antibody
Western Blot (WB), Immunoflorecence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
Source
Rabbit
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 58-170 of VHL (NP_000542.1).
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of U-251MG cells using VHL Polyclonal Antibody (AAA10644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U-251MG cells using VHL Polyclonal Antibody (AAA10644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human kidney using VHL Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human kidney using VHL Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using VHL antibody (AAA10644) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using VHL antibody (AAA10644) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-VHL antibody
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Product Categories/Family for anti-VHL antibody
References
Phosphorylation-dependent cleavage regulates von Hippel Lindau proteostasis and function. P German, S Bai, X-D Liu, M Sun, L Zhou, S Kalra, X Zhang, R Minelli, K L Scott, G B Mills, E Jonasch and Z Ding (2016)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated: 24kDa
Observed: 24kDa
Observed: 24kDa
NCBI Official Full Name
Von Hippel-Lindau disease tumor suppressor
NCBI Official Synonym Full Names
von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
NCBI Official Symbol
VHL
NCBI Official Synonym Symbols
RCA1; VHL1; pVHL; HRCA1
NCBI Protein Information
von Hippel-Lindau disease tumor suppressor; protein G7; elongin binding protein
UniProt Protein Name
Von Hippel-Lindau disease tumor suppressor
UniProt Gene Name
VHL
UniProt Entry Name
VHL_HUMAN
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Product Notes
The VHL vhl (Catalog #AAA10644) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VHL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoflorecence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200. Researchers should empirically determine the suitability of the VHL vhl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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