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IHC (Immunohistchemistry) (Vimentin Antibody-Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human small intestine tissue followed by peroxidase-conjugated secondary antibody and DAB staining.)

Rabbit anti-Mouse, Human Vimentin Polyclonal Antibody | anti-VIM antibody

Vimentin Rabbit anti-Human Polyclonal Antibody

Reactivity
Mouse, Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A purified
Synonyms
Vimentin; Polyclonal Antibody; Vimentin Rabbit anti-Human Polyclonal Antibody; IHC-plus Vimentin Antibody; VIM; vimenin; anti-VIM antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
Ig
Purity/Purification
Protein A purified
Form/Format
PBS, 0.09% Sodium Azide
Applicable Applications for anti-VIM antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Target
Human Vimentin
Conjugation
Unconjugated
Preparation and Storage
Maintain refrigerated at 2 degree C to 8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistchemistry)

(Vimentin Antibody-Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human small intestine tissue followed by peroxidase-conjugated secondary antibody and DAB staining.)

IHC (Immunohistchemistry) (Vimentin Antibody-Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human small intestine tissue followed by peroxidase-conjugated secondary antibody and DAB staining.)

IHC (Immunohistochemistry)

(Vimentin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (Vimentin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(Vimentin Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (Vimentin Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry)

(Vimentin Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (Vimentin Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(Vimentin Antibody-VIME Antibody western blot of HeLa,U251,A549,MDA-MB231 cell line lysates (35 ug/lane). The VIME antibody detected the VIME protein (arrow).)

WB (Western Blot) (Vimentin Antibody-VIME Antibody western blot of HeLa,U251,A549,MDA-MB231 cell line lysates (35 ug/lane). The VIME antibody detected the VIME protein (arrow).)

ICC (Immunocytochemistry)

(Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

ICC (Immunocytochemistry) (Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

ICC (Immunocytochemistry)

(Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

ICC (Immunocytochemistry) (Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

ICC (Immunocytochemistry)

(Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

ICC (Immunocytochemistry) (Vimentin Antibody-Confocal immunofluorescence of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry)

(Vimentin Antibody-Vimentin Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.)

FCM (Flow Cytometry) (Vimentin Antibody-Vimentin Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.)
Related Product Information for anti-VIM antibody
Vimentin antibody is an unconjugated rabbit polyclonal antibody to Vimentin from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,652 Da
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN

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Product Notes

The VIM vim (Catalog #AAA21381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vimentin Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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