Rabbit anti-Human VPS16 Polyclonal Antibody | anti-VPS16 antibody

Anti-VPS16 Antibody, Rabbit Polyclonal

Cat. Num. AAA256387

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: VPS16, Antibody; Anti-VPS16 Antibody, Rabbit Polyclonal; VPS16 Antibody, Rabbit PAb, Antigen Affinity Purified; vacuolar protein sorting 16 homolog (S. cerevisiae); Anti-hVPS16 Antibody; Anti-RP11-12M19.2 Antibody; anti-VPS16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VPS16
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-VPS16 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: E Coli-derived Human VPS16 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human VPS16 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(VPS16 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-VPS16 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-VPS16 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 95 kDaObserved band size :95 kDa)

WB (Western Blot)

(Anti-VPS16 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:95 kDaObserved band size:95 kDa)

Related Product Information for anti-VPS16 antibody

VPS16 (VPS16 Core Subunit Of CORVET And HOPS Complexes) is a Protein Coding gene. The VPS16 gene, located on 20p13, is conserved in the Rhesus monkey, dog, cow, mouse, and rat, etc. VPS16 belongs to the VPS16 family. 2 alternatively spliced human isoforms have been reported. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. VPS16 is widely expressed in the appendix, thyroid, and other tissues. Diseases associated with VPS16 include Dystonia and Hypophosphatasia, Infantile.

References

Yogosawa S, et al. (2005) Ubiquitylation and degradation of serum-inducible kinase by hvps18, a ring-h2 type ubiquitin ligase. J Biol Chem 280 (50): 41619-41627.Lin X, et al. (2014) Rilp interacts with hops complex via vps41 subunit to regulate endocytic trafficking. Sci Rep 4 7282.Kondo H, et al. (2017) Mutation in vps33a affects metabolism of glycosaminoglycans: A new type of mucopolysaccharidosis with severe systemic symptoms. Hum Mol Genet 26 (1): 173-183.