WB (Western Blot)
(Detection of human VTA1/LIP5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VTA1/LIP5 antibody AAA212965 (lot AAA212965-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human VTA1/LIP5 Polyclonal Antibody | anti-VTA1/LIP5 antibody
Rabbit anti-VTA1/LIP5 Antibody, Affinity Purified
Gene Names
VTA1; DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
VTA1/LIP5, Antibody; Rabbit anti-VTA1/LIP5 Antibody, Affinity Purified; LIP5; vesicle (multivesicular body) trafficking 1; vacuolar protein sorting-associated protein VTA1 homolog; SKD1-binding protein 1; SBP1; LYST-interacting protein 5; Vps20-associated 1 homolog; HSPC228; homolog of mouse SKD1-binding protein 1; DRG-1; DRG1; dopamine-responsive gene 1 protein; C6orf55; My012; Vacuolar protein sorting-associated protein VTA1 homolog; vesicle trafficking 1; anti-VTA1/LIP5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-VTA1/LIP5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 257 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human VTA1/LIP5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VTA1/LIP5 antibody AAA212965 (lot AAA212965-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human VTA1/LIP5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VTA1/LIP5 antibody AAA212965 (lot AAA212965-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human VTA1/LIP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VTA1/LIP5 antibody AAA212965 (lot AAA212965-1) used for IP at 6 ug per reaction. VTA1/LIP5 was also immunoprecipitated by rabbit anti-VTA1/LIP5 antibody For blotting immunoprecipitated VTA1/LIP5, AAA212965 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human VTA1/LIP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VTA1/LIP5 antibody AAA212965 (lot AAA212965-1) used for IP at 6 ug per reaction. VTA1/LIP5 was also immunoprecipitated by rabbit anti-VTA1/LIP5 antibody For blotting immunoprecipitated VTA1/LIP5, AAA212965 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-VTA1/LIP5 antibody
VTA1 is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VTA1 is thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. It is involved in the sorting and down-regulation of EGFR (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NP79].
Product Categories/Family for anti-VTA1/LIP5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36.5 kDa
NCBI Official Full Name
vacuolar protein sorting-associated protein VTA1 homolog isoform a
NCBI Official Synonym Full Names
vesicle (multivesicular body) trafficking 1
NCBI Official Symbol
VTA1
NCBI Official Synonym Symbols
DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
NCBI Protein Information
vacuolar protein sorting-associated protein VTA1 homolog
UniProt Protein Name
Vacuolar protein sorting-associated protein VTA1 homolog
UniProt Gene Name
VTA1
UniProt Synonym Gene Names
C6orf55; DRG-1; LIP5; SBP1
UniProt Entry Name
VTA1_HUMAN
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Product Notes
The VTA1/LIP5 vta1 (Catalog #AAA212965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-VTA1/LIP5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VTA1/LIP5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the VTA1/LIP5 vta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VTA1/LIP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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