SDS-PAGE
(3ug by SDS-PAGE under reducing conditions and can be visualized by coomassie blue stain.)
VTA1 recombinant protein
VTA1, 1-307aa, Human, His tag, E Coli
Gene Names
VTA1; DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
Reactivity
Human
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
VTA1; N/A; VTA1, 1-307aa, Human, His tag, E Coli; vacuolar protein sorting-associated protein VTA1 homolog; DRG-1; DRG1; LIP5; SBP1; VTA1 recombinant protein
Reactivity
Human
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAALAP LPPLPAQFKS IQHHLRTAQE HDKRDPVVAY YCRLYAMQTG MKIDSKTPEC RKFLSKLMDQ LEALKKQLGD NEAITQEIVG CAHLENYALK MFLYADNEDR AGRFHKNMIK SFYTASLLID VITVFGELTD ENVKHRKYAR WKATYIHNCL KNGETPQAGP VGIEEDNDIE ENEDAGAASL PTQPTQPSSS STYDPSNMPS GNYTGIQIPP GAHAPANTPA EVPHSTGVAS NTIQPTPQTI PAIDPALFNT ISQGDVRLTP EDFARAQKYC KYAGSALQYE DVSTAVQNLQ KALKLLTTGR E
Sequence Length
307
Applicable Applications for VTA1 recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
1-307aa
Preparation and Storage
Can be stored at 2°C to 8°C (1 week).
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing conditions and can be visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing conditions and can be visualized by coomassie blue stain.)
Related Product Information for VTA1 recombinant protein
VTA1 (Vps20-associated 1 homolog), also known as LIP5 (LYST-interacting protein 5), DRG1 (Dopamine-responsive protein DRG-1), SBP1 (SKD1 bindingprotein 1), belongs to the VTA1 family. This protein Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Also, VTA1 is known to interact with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3. Recombinant human VTA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for VTA1 recombinant protein
References
Ward D.M., et al. (2005) J. Biol. Chem. 280:10548-10555; Shim S., et al. (2008) Mol. Biol. Cell 19:2661-2672
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36.4kDa (331aa), confirmed by MALDI-TOF
NCBI Official Full Name
vacuolar protein sorting-associated protein VTA1 homolog isoform a
NCBI Official Synonym Full Names
vesicle trafficking 1
NCBI Official Symbol
VTA1
NCBI Official Synonym Symbols
DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
NCBI Protein Information
vacuolar protein sorting-associated protein VTA1 homolog
UniProt Protein Name
Vacuolar protein sorting-associated protein VTA1 homolog
UniProt Gene Name
VTA1
UniProt Synonym Gene Names
C6orf55; DRG-1; LIP5; SBP1
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Product Notes
The VTA1 vta1 (Catalog #AAA11823) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag!!Domain||1-307aa. The VTA1, 1-307aa, Human, His tag, E Coli reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VTA1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the VTA1 vta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMAALAP LPPLPAQFKS IQHHLRTAQE HDKRDPVVAY YCRLYAMQTG MKIDSKTPEC RKFLSKLMDQ LEALKKQLGD NEAITQEIVG CAHLENYALK MFLYADNEDR AGRFHKNMIK SFYTASLLID VITVFGELTD ENVKHRKYAR WKATYIHNCL KNGETPQAGP VGIEEDNDIE ENEDAGAASL PTQPTQPSSS STYDPSNMPS GNYTGIQIPP GAHAPANTPA EVPHSTGVAS NTIQPTPQTI PAIDPALFNT ISQGDVRLTP EDFARAQKYC KYAGSALQYE DVSTAVQNLQ KALKLLTTGR E. It is sometimes possible for the material contained within the vial of "VTA1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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