WB (Western Blot)
(Detection of human and mouse WARS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-WARS antibody AAA212587 (lot AAA212587-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse WARS Polyclonal Antibody | anti-WARS antibody
Rabbit anti-WARS Antibody, Affinity Purified
Gene Names
WARS; IFI53; IFP53; GAMMA-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
WARS, Antibody; Rabbit anti-WARS Antibody, Affinity Purified; hWRS; tryptophan--tRNA ligase, cytoplasmic; tryptophan tRNA ligase 1, cytoplasmic; trpRS; Tryptophanyl-tRNA synthetase; interferon-induced protein 53; HMN9; GAMMA-2; epididymis secretory sperm binding protein; IFP53; IFI53; Tryptophan--tRNA ligase, cytoplasmic; tryptophanyl-tRNA synthetase; anti-WARS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-WARS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse WARS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-WARS antibody AAA212587 (lot AAA212587-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse WARS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-WARS antibody AAA212587 (lot AAA212587-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human WARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WARS antibody AAA212587 (lot AAA212587-1) used for IP at 6 ug per reaction. WARS was also immunoprecipitated by rabbit anti-WARS antibody For blotting immunoprecipitated WARS, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human WARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WARS antibody AAA212587 (lot AAA212587-1) used for IP at 6 ug per reaction. WARS was also immunoprecipitated by rabbit anti-WARS antibody For blotting immunoprecipitated WARS, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human WARS by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-WARS (Cat. No. AAA212587 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human WARS by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-WARS (Cat. No. AAA212587 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-WARS antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family [taken from NCBI Entrez Gene (Gene ID: 7453)].
Product Categories/Family for anti-WARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,852 Da
NCBI Official Full Name
tryptophan--tRNA ligase, cytoplasmic isoform a
NCBI Official Synonym Full Names
tryptophanyl-tRNA synthetase
NCBI Official Symbol
WARS
NCBI Official Synonym Symbols
IFI53; IFP53; GAMMA-2
NCBI Protein Information
tryptophan--tRNA ligase, cytoplasmic; hWRS; interferon-induced protein 53; trpRS; tryptophan tRNA ligase 1, cytoplasmic
UniProt Protein Name
Tryptophan--tRNA ligase, cytoplasmic
UniProt Gene Name
WARS
UniProt Synonym Gene Names
IFI53; WRS; IFP53; TrpRS; hWRS
UniProt Entry Name
SYWC_HUMAN
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Product Notes
The WARS wars (Catalog #AAA212587) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-WARS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WARS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the WARS wars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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