Rabbit anti-Human WBSCR22 Polyclonal Antibody | anti-BUD23 antibody

Anti-WBSCR22 Antibody, Rabbit Polyclonal

Cat. Num. AAA256044

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: WBSCR22, Antibody; Anti-WBSCR22 Antibody, Rabbit Polyclonal; WBSCR22 Antibody, Rabbit PAb, Antigen Affinity Purified; Williams Beuren syndrome chromosome region 22; Anti-HASJ4442 Antibody; Anti-HUSSY-3 Antibody; Anti-MERM1 Antibody; Anti-PP3381 Antibody; Anti-WBMT Antibody; anti-BUD23 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human WBSCR22
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-BUD23 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human WBSCR22 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human WBSCR22 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(WBSCR22 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-WBSCR22 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-WBSCR22 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human WBSCR22 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human WBSCR22 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-WBSCR22 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-BUD23 antibody

One of the proteins involved in modifying ribosomes so they are functional is called BUD23. BUD23 in Saccharomyces cerevisiae encodes for a class I methyltransferase, and deletion of the gene results in slow growth and random budding phenotypes. Deletion of BUD23 leads to reduced levels of the small (40S) ribosomal subunit, and a block in processing 20S rRNA to 18S rRNA, a late step in 40S maturation. Bud23 protein, but not its methyltransferase activity, is important for biogenesis and export of the 40S subunit in yeast. Baxter et al. found that ribosomes in human cells with no BUD23 were different than in normal cells and that cells without BUD23 produced different proteins, which did not always perform their roles correctly. Proteins in the mitochondria are one of the main groups affected by the absence of BUD23.

References

White J, et al. (2008) Bud23 methylates g1575 of 18s rrna and is required for efficient nuclear export of pre-40s subunits. Mol Cell Biol 28 (10): 3151-3161.Lin JL, et al. (2012) New phenotypes generated by the g57r mutation of bud23 in saccharomyces cerevisiae. Yeast 29 (12): 537-546.Baxter M, et al. (2020) Cardiac mitochondrial function depends on bud23 mediated ribosome programming. Elife 9: e50705.