WB (Western Blot)
(Detection of human WIPF1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WIPF1 antibody AAA213237 (lot AAA213237-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human WIPF1 Polyclonal Antibody | anti-WIPF1 antibody
Rabbit anti-WIPF1 Antibody, Affinity Purified
Gene Names
WIPF1; WIP; WAS2; PRPL-2; WASPIP
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
WIPF1, Antibody; Rabbit anti-WIPF1 Antibody, Affinity Purified; WAS/WASL-interacting protein family member 1; WASPIP; Wiskott-Aldrich syndrome protein-interacting protein; Wiskott-Aldrich syndrome protein interacting protein; WIP; WAS2; protein PRPL-2; testicular tissue protein Li 226; PRPL-2; WASP-interacting protein; WAS/WASL interacting protein family member 1; anti-WIPF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-WIPF1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human WIPF1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WIPF1 antibody AAA213237 (lot AAA213237-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human WIPF1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WIPF1 antibody AAA213237 (lot AAA213237-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human WIPF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPF1 antibody AAA213237 (lot AAA213237-1) used for IP at 6 ug per reaction. WIPF1 was also immunoprecipitated by rabbit anti-WIPF1 antibody BL21323. For blotting immunoprecipitated WIPF1, AAA213237 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human WIPF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPF1 antibody AAA213237 (lot AAA213237-1) used for IP at 6 ug per reaction. WIPF1 was also immunoprecipitated by rabbit anti-WIPF1 antibody BL21323. For blotting immunoprecipitated WIPF1, AAA213237 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-WIPF1 antibody
WAS/WASL-interacting protein family member 1 (WIPF1) plays an important role in the organization of the actin cytoskeleton. WIPF1 binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the diseae [taken from NCBI Entrez Gene (Gene ID: 7456)].
Product Categories/Family for anti-WIPF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,426 Da
NCBI Official Full Name
WAS/WASL-interacting protein family member 1
NCBI Official Synonym Full Names
WAS/WASL interacting protein family member 1
NCBI Official Symbol
WIPF1
NCBI Official Synonym Symbols
WIP; WAS2; PRPL-2; WASPIP
NCBI Protein Information
WAS/WASL-interacting protein family member 1
UniProt Protein Name
WAS/WASL-interacting protein family member 1
UniProt Gene Name
WIPF1
UniProt Synonym Gene Names
WASPIP; WIP; WASP-interacting protein
UniProt Entry Name
WIPF1_HUMAN
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Product Notes
The WIPF1 wipf1 (Catalog #AAA213237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-WIPF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WIPF1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the WIPF1 wipf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WIPF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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