Application Data
(Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Rabbit WNT-3a Polyclonal Antibody | anti-WNT-3a antibody
RABBIT ANTI HUMAN WNT-3a:Biotin
Applications
Western Blot, ELISA
Synonyms
WNT-3a, Antibody; RABBIT ANTI HUMAN WNT-3a:Biotin; anti-WNT-3a antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Biotin
Purified IgG conjugated to Biotin - lyophilised
Purified IgG conjugated to Biotin - lyophilised
Concentration
IgG concentration 0.1mg/ml after reconstitution (varies by lot)
Sequence Length
385
Applicable Applications for anti-WNT-3a antibody
WB (Western Blot), ELISA
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Immunogen
Recombinant human WNT-3a
Buffer Solution
Buffer Solution
Antiserum Preparation
Antiserum to human WNT-3a were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formlin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human WNT-3a following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Related Product Information for anti-WNT-3a antibody
Rabbit anti Human WNT-3a antibody specifically recognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, which acts as a ligand for members of the frizzled family of seven transmembrane receptors, and plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a has been shown to act upstream of the Notch signalling pathway, in the regulation of somitogenesis, which is believed to be controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,905 Da
NCBI Official Full Name
protein Wnt-3a
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol
WNT3A
NCBI Protein Information
protein Wnt-3a
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
WNT3A
UniProt Entry Name
WNT3A_HUMAN
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Product Notes
The WNT-3a wnt3a (Catalog #AAA50093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WNT-3a can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the WNT-3a wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT-3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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