Application Data
(Paraformaldehyde-fixed, para fin embedded (Rat brain);Antigen retrieval by boiling in sodium citrate bu fer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking bu fer (normal goat serum) at 37? for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4?, fo lowed by operating according to SP Kit (Rabbit) instructions and DAB staining.)
Rabbit WNT7A Polyclonal Antibody | anti-WNT7A antibody
WNT7A
Reactivity
Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity purified by Protein A
Synonyms
WNT7A, Antibody; WNT7A; Protein Wnt-7a; wnt 7a;Protein Wnt-7a precursor; proto-oncogene wnt7a protein; wingless-type MMTV integration site family; member 7A; WNT7A_HUMAN; anti-WNT7A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified by Protein A
Form/Format
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-WNT7A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human WNT7A 241-349/349
Positive Control
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
Subcellular location
Secreted, extracellular space, extracellular matrix.
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.
Application Data
(Paraformaldehyde-fixed, para fin embedded (Rat brain);Antigen retrieval by boiling in sodium citrate bu fer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking bu fer (normal goat serum) at 37? for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4?, fo lowed by operating according to SP Kit (Rabbit) instructions and DAB staining.)
Application Data
(Paraformaldehyde-fixed, para fin embedded (Rat brain);Antigen retrieval by boiling in sodium citrate bu fer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking bu fer (normal goat serum) at 37? for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4?, fo lowed by operating according to SP Kit (Rabbit) instructions and DAB staining.)
WB (Western Blot)
(Brain (Mouse) Lysate at 40 ug. Primary: Anti-WNT7A at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 41 kD. Observed band size: 50 kD.)
WB (Western Blot)
(Brain (Mouse) Lysate at 40 ug. Primary: Anti-WNT7A at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 41 kD. Observed band size: 50 kD.)
Related Product Information for anti-WNT7A antibody
Protein Wnt-7a is a protein that in humans is encoded by the WNT7A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein showing 99% amino acid identity to the mouse Wnt7A protein. This gene not only guides the development of the anterior-posterior axis in the female reproductive tract but also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. It is also responsive to changes in the levels of sex steroid hormone in the female reproductive tract. Decreased expression of this gene in human uterine leiomyoma is found to be inversely associated with the expression of estrogen receptor alpha.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
41 kDa
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Product Notes
The WNT7A (Catalog #AAA269019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT7A reacts with Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's WNT7A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT7A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT7A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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