FCM/FACS (Flow Cytometry)
(WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human WRN Polyclonal Antibody | anti-WRN antibody
WRN Antibody (Center T802)
Gene Names
WRN; RECQ3; RECQL2; RECQL3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
WRN, Antibody; WRN Antibody (Center T802); Werner syndrome ATP-dependent helicase; DNA helicase; RecQ-like type 3; RecQ3; Exonuclease WRN; 31--; RecQ protein-like 2; WRN; RECQ3; RECQL2; anti-WRN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This WRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human WRN.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
787-816
Sequence Length
1,432
Applicable Applications for anti-WRN antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(WRN Antibody (Center T802) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(WRN Antibody (Center T802) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).)
WB (Western Blot)
(WRN Antibody (Center T802) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the WRN antibody detected the WRN protein (arrow).)
Related Product Information for anti-WRN antibody
This gene encodes a member of the RecQ subfamily and the
DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA
helicases are involved in many aspects of DNA metabolism, including
transcription, replication, recombination, and repair. This protein
contains a nuclear localization signal in the C-terminus and shows
a predominant nucleolar localization. It possesses an intrinsic 3'
to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.
Based on interactions between this protein and Ku70/80 heterodimer
in DNA end processing, this protein may be involved in the repair
of double strand DNA breaks. Defects in this gene are the cause of
Werner syndrome, an autosomal recessive disorder characterized by
premature aging.
DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA
helicases are involved in many aspects of DNA metabolism, including
transcription, replication, recombination, and repair. This protein
contains a nuclear localization signal in the C-terminus and shows
a predominant nucleolar localization. It possesses an intrinsic 3'
to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.
Based on interactions between this protein and Ku70/80 heterodimer
in DNA end processing, this protein may be involved in the repair
of double strand DNA breaks. Defects in this gene are the cause of
Werner syndrome, an autosomal recessive disorder characterized by
premature aging.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Perry, J.J., et al. J. Biol. Chem. 285(33):25699-25707(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Kobayashi, J., et al. Mech. Ageing Dev. 131(6):436-444(2010)
Ehrenberg, M., et al. Mol. Vis. 16, 1771-1775 (2010) :
Perry, J.J., et al. J. Biol. Chem. 285(33):25699-25707(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Kobayashi, J., et al. Mech. Ageing Dev. 131(6):436-444(2010)
Ehrenberg, M., et al. Mol. Vis. 16, 1771-1775 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162461
NCBI Official Full Name
Werner syndrome ATP-dependent helicase
NCBI Official Synonym Full Names
Werner syndrome, RecQ helicase-like
NCBI Official Symbol
WRN
NCBI Official Synonym Symbols
RECQ3; RECQL2; RECQL3
NCBI Protein Information
Werner syndrome ATP-dependent helicase
UniProt Protein Name
Werner syndrome ATP-dependent helicase
UniProt Gene Name
WRN
UniProt Synonym Gene Names
RECQ3; RECQL2; RecQ3
UniProt Entry Name
WRN_HUMAN
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Product Notes
The WRN wrn (Catalog #AAA286699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 787-816. The WRN Antibody (Center T802) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WRN can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WRN wrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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