WB (Western Blot)
(Detection of human WRN by western blot. Samples: Whole cell lysate (10 ug) from K-562, RKO, LNCaP, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WRN antibody (AAA210835 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human WRN Polyclonal Antibody | anti-WRN antibody
Rabbit anti-WRN Antibody, Affinity Purified
Gene Names
WRN; RECQ3; RECQL2; RECQL3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
WRN, Antibody; Rabbit anti-WRN Antibody, Affinity Purified; exonuclease WRN; RECQL3; Werner syndrome, RecQ helicase-like; Werner syndrome ATP-dependent helicase; DNA helicase, RecQ-like type 3; recQ protein-like 2; RECQ3; RECQL2; Werner syndrome RecQ like helicase; anti-WRN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-WRN antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1382 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human WRN by western blot. Samples: Whole cell lysate (10 ug) from K-562, RKO, LNCaP, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WRN antibody (AAA210835 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human WRN by western blot. Samples: Whole cell lysate (10 ug) from K-562, RKO, LNCaP, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WRN antibody (AAA210835 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human WRN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WRN antibody AAA210835 (lot AAA210835-3) used for IP at 6 ug per reaction. WRN was also immunoprecipitated by a previous lot of this antibody (lot AAA210835) and rabbit anti-WRN antibody Brother. For blotting immunoprecipitated WRN, AAA210835 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human WRN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WRN antibody AAA210835 (lot AAA210835-3) used for IP at 6 ug per reaction. WRN was also immunoprecipitated by a previous lot of this antibody (lot AAA210835) and rabbit anti-WRN antibody Brother. For blotting immunoprecipitated WRN, AAA210835 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-WRN antibody
The protein product of the Werner's syndrome (WRN) gene is the Werner syndrome ATP-dependent helicase. WRN is part of the ReqQ family of helicases that unwind DNA in preparation for replication, transcription, and repair. WRN appears to play a versatile role in cellular processes including DNA replication, transcription and the maintenance of genomic integrity.
Product Categories/Family for anti-WRN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162,461 Da
NCBI Official Full Name
Werner syndrome ATP-dependent helicase
NCBI Official Synonym Full Names
Werner syndrome, RecQ helicase-like
NCBI Official Symbol
WRN
NCBI Official Synonym Symbols
RECQ3; RECQL2; RECQL3
NCBI Protein Information
Werner syndrome ATP-dependent helicase; exonuclease WRN; recQ protein-like 2; DNA helicase, RecQ-like type 3
UniProt Protein Name
Werner syndrome ATP-dependent helicase
UniProt Gene Name
WRN
UniProt Synonym Gene Names
RECQ3; RECQL2; RecQ3
UniProt Entry Name
WRN_HUMAN
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Product Notes
The WRN wrn (Catalog #AAA210835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-WRN Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WRN can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the WRN wrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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