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Rabbit anti-Human, Mouse XBP-1 Polyclonal Antibody | anti-XBP1 antibody
XBP-1 Antibody
Gene Names
XBP1; XBP2; TREB5; XBP-1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
XBP-1 Antibody is affinity chromatography purified via peptide column.
Synonyms
XBP-1, Antibody; XBP-1 Antibody; XBP-1; XBP2; TREB5; X-box-binding protein 1; Tax-responsive element-binding protein 5; X-box binding protein 1; anti-XBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
XBP-1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
261
Applicable Applications for anti-XBP1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 ug/mL. Antibody can also be
used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
Optimal dilutions for each application to be determined by the researcher.
Optimal dilutions for each application to be determined by the researcher.
Conjugate
Unconjugated
Immunogen
XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1.
Buffer
XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)
Preparation and Storage
XBP-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of XBP-1 in rat liver tissue with XBP-1 Antibody at 20 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of XBP-1 in rat liver tissue with XBP-1 Antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of XBP1 expression in rat liver tissue lysate with XBP1 antibody at (A) 1 and (B) 2 μg/ml.)
IHC (Immunohistchemistry)
(Immunohistochemistry of XBP1 in mouse liver tissue with XBP1 antibody at 2 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of XBP1 in human liver tissue with XBP1 antibody at 20 μg/ml.Green: XBP-1 Antibody (3687)Blue: DAPI staining)
IHC (Immunohistochemistry)
(Immunohistochemistry of XBP1 in human liver tissue with XBP1 antibody at 5 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of XBP-1 in HepG2 cells with XBP-1 antibody at 20 μg/ml.Green: XBP-1 Antibody (3687)Blue: DAPI staining)
ICC (Immunocytochemistry)
(Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 10 μg/mL.)
WB (Western Blot)
(Western blot analysis of XBP-1 in HepG2 cell lysate with XBP-1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide)
Related Product Information for anti-XBP1 antibody
XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,148 Da
NCBI Official Full Name
X-box-binding protein 1
NCBI Official Synonym Full Names
X-box binding protein 1
NCBI Official Symbol
XBP1
NCBI Official Synonym Symbols
XBP2; TREB5; XBP-1
NCBI Protein Information
X-box-binding protein 1; tax-responsive element-binding protein 5
UniProt Protein Name
X-box-binding protein 1
UniProt Gene Name
XBP1
UniProt Synonym Gene Names
TREB5; XBP2; XBP-1
UniProt Entry Name
XBP1_HUMAN
