WB (Western Blot)
(Fig: 2 Western blot analysis of XIAP in various tumor cell lines using at 1: 2000.)
Rabbit XIAP Polyclonal Antibody | anti-XIAP antibody
Polyclonal Antibody to XIAP
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Gerbil, Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Serum
Synonyms
XIAP, Antibody; Polyclonal Antibody to XIAP; XIAP; API3; BIRC4; IAP3; Baculoviral IAP repeat-containing protein 4; ILP; IAP-like protein; IAP-3; Inhibitor of apoptosis protein 3; X-linked IAP; X-linked inhibitor of apoptosis protein; Antibody to XIAP; Anti-XIAP Antibody; XIAP Antibody; anti-XIAP antibody
Host
Rabbit
Reactivity
Gerbil, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
497
Applicable Applications for anti-XIAP antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Information
Recombinant BIR2 domain protein fragment of human XIAP was used as immunogen for this antibody
Antigen
XIAP
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
WB (Western Blot)
(Fig: 2 Western blot analysis of XIAP in various tumor cell lines using at 1: 2000.)
WB (Western Blot)
(Fig: 2 Western blot analysis of XIAP in various tumor cell lines using at 1: 2000.)
WB (Western Blot)
(Fig: 1 Western blot analysis of XIAP using in human embryonic kidney 293 cells using at 1: 2000.)
WB (Western Blot)
(Fig: 1 Western blot analysis of XIAP using in human embryonic kidney 293 cells using at 1: 2000.)
Related Product Information for anti-XIAP antibody
XIAP [human X-linked IAP, hILP (human IAP-like protein), MIHA, BIRC4) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including XIAP, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination (Morizane et al, 2005). Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. XIAP is highly characterized with respect to its structure and biochemical mechanisms, and has received interest as a therapeutic target (reviewed in Schimmer, 2006). Since XIAP blocks a substantial portion of the apoptosis pathway and is associated with chemoresistance in cancer cells, inhibiting XIAP has been a focus for potential therapeutics. Approaches have included antisense oligonucleotides and small molecule inhibitors. Small molecules that that target the BIR2 and BIR3 domains of XIAP are considered particularly attractive. This is because the BIR domains inhibit caspase activity, and it is thought that removing the inhibition should increase the cell's ability to undergo apoptosis as well as decrease its potential for chemoresistance. This antibody recognizes XIAP. Full-length human XIAP is a 497 amino acid protein and migrates at approx. 53 kDa on SDS-PAGE.
Product Categories/Family for anti-XIAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,685 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase XIAP
NCBI Official Synonym Full Names
X-linked inhibitor of apoptosis
NCBI Official Symbol
XIAP
NCBI Official Synonym Symbols
API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
NCBI Protein Information
E3 ubiquitin-protein ligase XIAP
UniProt Protein Name
E3 ubiquitin-protein ligase XIAP
UniProt Gene Name
XIAP
UniProt Synonym Gene Names
API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3; X-linked IAP
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Product Notes
The XIAP xiap (Catalog #AAA78369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to XIAP reacts with Gerbil, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XIAP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the XIAP xiap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XIAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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