IF (Immunofluorescence)
(Immunofluorescence staining of XPNPEP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPNPEP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human XPNPEP1 Polyclonal Antibody | anti-XPNPEP1 antibody
Anti-XPNPEP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
XPNPEP1, Antibody; Anti-XPNPEP1 Antibody, Rabbit Polyclonal; XPNPEP1 Antibody, Rabbit PAb, Antigen Affinity Purified; X-prolyl aminopeptidase (aminopeptidase P) 1, soluble; Anti-APP1 Antibody; Anti-SAMP Antibody; Anti-XPNPEP Antibody; Anti-XPNPEPL Antibody; Anti-XPNPEPL1 Antibody; anti-XPNPEP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human XPNPEP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-XPNPEP1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human XPNPEP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human XPNPEP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of XPNPEP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPNPEP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of XPNPEP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XPNPEP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot)
(Anti-XPNPEP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: A549 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-XPNPEP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: A549 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-XPNPEP1 antibody
XPNPEP1 (X-Prolyl Aminopeptidase 1, also known as APP1) is a Protein Coding gene. The XPNPEP1 gene, located on 10q25.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes the cytosolic form of a Metallo-Aminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in the degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Deficiency of aminopeptidase P1, a cytosolic proline-specific peptidase encoded by the Xpnpep1 gene, causes an inborn error of metabolism (IEM) characterized by peptiduria in humans. XPNPEP1 is widely expressed in the small intestine, duodenum, and other tissues. Diseases associated with XPNPEP1 include Biliary Atresia and Parkinson's Disease 4, Autosomal Dominant.
References
Li X, et al. (2008) Structure of human cytosolic x-prolyl aminopeptidase: A double mn(ii)-dependent dimeric enzyme with a novel three-domain subunit. J Biol Chem 283 (33): 22858-22866.Iyer S, et al. (2015) Crystal structure of x-prolyl aminopeptidase from caenorhabditis elegans: A cytosolic enzyme with a di-nuclear active site. FEBS Open Bio 5 292-302.Bae YS, et al. (2018) Deficiency of aminopeptidase p1 causes behavioral hyperactivity, cognitive deficits, and hippocampal neurodegeneration. Genes Brain Behav 17 (2): 126-138.
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Product Notes
The XPNPEP1 (Catalog #AAA256504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-XPNPEP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XPNPEP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the XPNPEP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XPNPEP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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